Abstract The identification of a “new” handwriting Manuel Chrysoloras allows to assign him number hitherto unpublished witnesses, all preceding his teaching activity in Florence from 1397. This new dossier illustrates Chrysoloras’ youth Constantinople, providing precious and so far unknown information on family, father John, studies the anti-Palamite milieux with Isaac Argyros Demetrios Kydones...

win a spot in Mass General’s surgical residency. As a resident in surgery and later a fellow in cardiac surgery, he was known to arrive at 3 AM to prepare for his clinical responsibilities. Even decades later, his contemporaries---many of them now senior surgical staff at Mass General---still speak of how he always prioritized the care of the patient, and how he never left work unfinished for h...

First of all, I would like to express gratitude Prof. Marian P. Kaźmierkowski for the continuous support and help during work of the thesis. His precious advice and numerous discussions enhanced my knowledge and scientific inspiration. Finally, I would like thank to my whole family, particularly my parents for their love and patience.

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydr...

The cDNA of the human GM2-activator protein was cloned into the expression vector pHX17. The plasmid encodes a fusion protein with a hexahistidine tail and a Factor Xa cleavage site at its N-terminus. The recombinant protein was purified from cell homogenates under denaturing conditions by metal-ion affinity chromatography in a single step and then was refolded. The hexahistidine tail could be ...

We report oriented immobilization of proteins using the standard hexahistidine (His6)-Ni2+:NTA (nitrilotriacetic acid) methodology, which we systematically tuned to give control of surface coverage. Fluorescence microscopy and surface plasmon resonance measurements of self-assembled monolayers (SAMs) of red fluorescent proteins (TagRFP) showed that binding strength increased by 1 order of magni...