There have been a number of cases of foodborne illness among humans that are caused by pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, etc. The current practices to detect such pathogenic agents are cell culturing, immunoassays, or polymerase chain reactions (PCRs). These methods are essentially laboratory-based methods that are not at all real-time and thus unavailable for ...

The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply 'Lab on a chip' systems, have witnessed steady advances over the last several years. Here, we summarize recent research on chip substrates, surface treatments, ...

In this paper we present a modular lab-on-a-chip system for integrated sample pre-treatment (PT) by magnetophoresis and DNA amplification by polymerase chain reaction (PCR). It consists of a polymer-based microfluidic chip mounted on a custom-made thermocycler (Figure 1) and includes a simple and efficient method for switching the liquid flow between the PT and PCR chamber. Purification of huma...

In this paper, a lab-on-chip system dedicated to fungi cultivation in Earth’s gravity and simulated microgravity, being solution that could be used future nanosatellite missions, is shown. For the first time, fully glass structure enabling proper environment for of species—Fusarium culmorum—is presented. Apart from biological validation cultures with use system, tests were carried out under ind...