Marine carbon and nitrogen processing through microorganisms’ metabolism is an important aspect of the global element cycles. For that purpose, we used foraminifera to analyze turnover with different algae food sources. In Baltic Sea, benthic are quite common therefore it understand their metabolism. Especially, Cribroelphidium selseyense , also occurring in has often been for laboratory feedin...

In normal growth conditions, total protein percent (salt soluble plus hordein fractions) in the endosperm at maturity in barley cultivar Hordeum vulgare L. cv ;Ruth' was about 14%, whereas in an accession of wild barley, Hordeum spontaneum Koch line 297, it was about 28%. Spike culture experiments were conducted to ascertain whether there were basic differences between the two genotypes under c...

HetR is a serine-type protease required for heterocyst differentiation in heterocystous cyanobacteria under conditions of nitrogen deprivation. We have identified the active Ser residue of HetR from Anabaena sp. strain PCC 7120 by site-specific mutagenesis. By changing the S152 residue to an Ala residue, the mutant protein cannot be labeled by Dansyl fluoride, a specific serine-type protein inh...

l-Canavanine, the guanidinooxy structural analog of l-arginine, is an important nonprotein amino acid of many leguminous plants with nitrogen storage a major proported role. l-[Guanidinooxy-(14)C]canavanine, [(14)C] urea, and [(15)N]urea were injected separately into the fleshy, green cotyledons of 9-day old jack bean plants, Canavalia ensiformis (L.) DC. [Leguminosae]. There was significant tr...

We performed a 15N-labeling study to investigate nitrogen incorporation and retention by the benthic microbial community (bacteria and benthic microalgae) and fauna in the intertidal sediment of the subtropical Australian Brunswick Estuary. The main experiment involved an in situ 15N pulse–chase experiment. After injection of 15NH z4 into the sediment, 15N was traced into bulk sediment, total h...

BACKGROUND The in vivo quality of milk protein fractions has seldom been studied in humans. OBJECTIVE Our objective was to compare the postprandial utilization of dietary nitrogen from 3 [(15)N]-labeled milk products: micellar caseins (MC), milk soluble protein isolate (MSPI), and total milk protein (TMP). DESIGN The macronutrient intakes of 23 healthy volunteers were standardized for 1 wk,...

We describe the use of carbon stable isotope ((13)C) labeled n-alkanes as a potential internal tracer to assess passage kinetics of ingested nutrients in ruminants. Plant cuticular n-alkanes originating from intrinsically (13)C labeled ryegrass plants were pulse dosed intraruminally in four rumen-cannulated lactating dairy cows receiving four contrasting ryegrass silage treatments that differed...

The use of labeled butanol for autoradiographic and positron tomographic measurement of cerebral blood flow has been well established using radiocarbon labels. The advantages of the short half-life of oxygen-15 (15O) in doing sequential flow studies are also recognized. An automated procedure has been developed for the routine rapid and sequential synthesis of 15O-labeled butanol in amounts and...