PURPOSE To analyze the resistance mechanisms in Acinetobacter species by phenotypic methods. METHODS Antibiotic susceptibility profile for 150 clinical isolates of Acinetobacte r was determined by the standard disk diffusion method. Isolates detected to be meropenem resistant were tested further by broth microdilution minimum inhibitory concentration (MIC) for meropenem. The resistant isolate...

This study was designed to evaluate the performance of the broth microdilution (BMD) test in detecting the production of Klebsiella pneumoniae carbapenemases (KPCs) and metallo-β-lactamases (MBLs) in clinical isolates of gram-negative bacilli by using various β-lactamase inhibitors. A carbapenemase detection test, comprised of Mueller-Hinton broth containing serial twofold dilutions of imipenem...

New Delhi metallo-β-lactamase 1 (NDM-1) is a novel type of metallo-β-lactamase (MBL). Enterobacteriaceae carrying this NDM-1 encoding gene, bla(NDM-1), have been identified worldwide. Bacteria carrying bla(NDM-1) are not only resistant to carbapenem, but also highly resistant to many classes of antibiotics, which indicate the importance of prompt identification of these bacteria and implementat...

The metallo-β-lactamase (MBL) GOB-1 was expressed via a T7 expression system in Escherichia coli BL21(DE3). The MBL was purified to homogeneity and shown to exhibit a broad substrate profile, hydrolyzing all the tested β-lactam compounds efficiently. The GOB enzymes are unique among MBLs due to the presence of a glutamine residue at position 116, a zinc-binding residue in all known class B1 and...

We evaluated the ability of the combination disk test (CDT) and the Modified Hodge Test (MHT) to discriminate between various carbapenemase-producing Pseudomonas aeruginosa isolates (KPC, n = 36; metallo-β-lactamase (MBL), n = 38) and carbapenemase non-producers (n = 75). For the CDT, the optimal inhibitor concentrations and cut-off values were: 600 μg of 3-aminophenylboronic acid (APB) per dis...

We conducted a study on molecular epidemiology and clinical implications of metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa isolated from urine. Over a 10-year period from 2001 through 2010, a total of 92 MBL-producing P. aeruginosa urine isolates were collected from patients (one isolate per patient) who were admitted to 5 hospitals in Okayama Prefecture, Japan. When cross-infect...

Carbapenem resistance due to acquired metallo-beta-lactamases (MBLs) is considered to be more serious than other resistance mechanisms. The aim of this study was to evaluate the antibacterial activity of Zataria multiflora Boiss and Carum copticum plants on IMP-producing P.aeruginosa strains. This experimental study was carried out on hospitalized burn patients during 2011 and 2012. Antibiotics...

Background: Emergence of carbapenemase producing microorganisms, specifically the New Delhi metallo-β-lactamase (NDM) and its different groups, in areas all over world, raised a global health concern. metallo-beta-lactamase destructs carbapenems, that are considered one last lines treatment for infections caused by resistant strains. Aim: Our study aimed detection NDM gene among carbapenem Kleb...

Klebsiella pneumoniae is a pathogen primarily capable of causing urinary tract infections and pneumonia in otherwise healthy people. Most of the strains are frequently resistant to numerous antibiotics, responsible for conferring multidrug resistance. This study was conducted to determine the prevalence of extended spectrum β-lactamases (ESBL), AmpC β-lactamases and metallo β-lactamases (MBL) a...

BACKGROUND Twenty four non replicate imipenem resistant P. aeruginosa were isolated between January and November 2008, in the kidney transplantation unit of Charles Nicolle Hospital of Tunis (Tunisia). This study was conducted in order to establish epidemiological relationship among them and to identify the enzymatic mechanism involved in imipenem resistance. METHODS Analysis included antimic...