The effect of dolichol C(95) on the structure and thermotropic phase behaviour of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine and stearoyloleoylphosphatidylethanolamine has been examined by synchrotron X-ray diffraction and differential scanning calorimetry. The presence of dolichol C(95) had no detectable effects on the temperature of either the gel to ripple or the rip...

lamellar ichthyosis is one form of congenital autosomal recessive ichthyosis. to date, seven causative genes for arci have been identified. to understand further the genetic spectrum of the disease, we analyzed a four-generation iranian family with arci that had observable inheritance. exome sequencing data for one of the affected individuals with ichthyosis from a consanguineous iranian family...

purpose: to evaluate the influence of donor characteristics (age, death-to-preservation time, preservation-to-surgery time, graft rating, and endothelial cell features) on clinical outcomes following deep anterior lamellar keratoplasty (dalk) in patients with keratoconus. methods: in this retrospective study, 290 consecutive keratoconic eyes underwent dalk using donor corneas of different quali...

purpose: to compare the clinical and paraclinical outcomes following deep anterior lamellar keratoplasty (dalk) using frozen versus fresh corneas in patients with keratoconus. methods: in this prospective, comparative interventional study, 16 frozen and 19 fresh corneas were used during dalk in keratoconus patients. big bubble technique was used in all patients. the two groups were compared at ...

purpose: to report a case of bilateral acanthamoeba keratitis with recurrence after keratoplasties case report: here we report a case with bilateral acanthamoeba keratitis who initially responds to medical treatment, after discontinuation of treatment the disease flared up and not responded to medical therapy so superficial keratectomy was done and medical treatment continued and the ulcer impr...

objective autosomal recessive congenital ichthyosis (arci) is a rare, heterogenous keratinization disorder of the skin, classically divided into two clinical subtypes, lamellar ichthyosis (li) and nonbullous congenital ichthyosi-formis erythroderma (ncie). lamellar ichtyosis is caused by mutations in the tgm1 gene that encodes transglutaminase 1 enzyme, which is critical for the assembly of the...

Surfactant protein B is essential for normal pulmonary surfactant activity and lamellar body genesis in type 2 cells. However, the role of SP-B in lamellar body genesis is poorly understood. We developed an adenovirus vector expressing antisense SP-B as an alternative in vitro model of SP-B deficiency to begin to explore the role of SP-B in lamellar body genesis. RT-PCR analysis revealed that a...

The internal pH of lung lamellar bodies was investigated with membrane permeable basic amines. Isolated granular pneumocytes and isolated lung lamellar bodies exhibited fluorescence when exposed to 8 microM quinacrine, suggesting accumulation of this dye due to an acidic internal pH. Uptake of [14C]methylamine by isolated lung lamellar bodies was measured to quantitate the intralamellar body pH...

We use a coarse-grained molecular model to study the self-assembly process of complexes of cationic and neutral lipids with DNA molecules (‘‘lipoplexes’’)—a promising nonviral carrier of DNA for gene therapy. We identify the resulting structures through direct visualization of the molecular arrangements and through calculations of the corresponding scattering plots. The latter approach provides...