نتایج جستجو برای: lamp pcr
تعداد نتایج: 184386 فیلتر نتایج به سال:
Typhoid fever remains a public health threat in many countries. A positive result in traditional culture is a gold-standard for typhoid diagnosis, but this method is time consuming and not sensitive enough for detection of samples containing a low copy number of the target organism. The availability of the loop-mediated isothermal amplification (LAMP) assay, which offers high speed and simplici...
The aim of the study was to compare Polymerase Chain Reaction (PCR) and Loop Mediated Isothermal Amplification (LAMP) assay for detection Salmonella in Chicken Meat. Food safety remains a major concern worldwide, with outbreaks foodborne illness resulting significant costs individuals food industry. genus is most prevalent group pathogens that are regularly isolated from food-producing animals ...
We compared the performance of a recently established real-time loop-mediated amplification (LAMP) assay with the one from a highly sensitive quantitative PCR assay. None of these assays produced false-positive results in this study. For samples isolated from patients within the first 3 days of disease onset, the detection rate of the quantitative PCR assay was higher (14 of 15 were positive) t...
چکیده زمینه و هدف: ویروس هرپس سیمپلکس تیپ 1 و 2 عامل ایجاد عفونت سیستم عصبی مرکزی (آنسفالیت) در انسان می باشد. روشهای مولکولی، بهترین روش تشخیص این عامل بیماریزا می باشند. در این مطالعه تکنیک مولکولی جدید lamp جهت تشخیص هر دو تیپ این ویروس مورد ارزیابی قرار گرفت. روش بررسی: در یک مطالعه تجربی، 184 نمونه مایع مغزی- نخاعی (csf) از بیمارستان مفید تهران تهیه شد. dna نمونه ها بوسیله کیت dnp سیناژن ا...
BACKGROUND Cystic echinococcosis is highly prevalent in northwest China. A cost-effective, easy to operate diagnostic tool with high sensitivity and specificity would greatly facilitate the monitoring of Echinococcus infections in canine definitive hosts. METHODS The primers used in the LAMP assay were based on the mitochondrial nad5 gene of E. granulosus sensu stricto (E. granulosus s.s., or...
Highly sensitive and field deployable molecular diagnostic tools are critically needed for detecting submicroscopic, yet transmissible levels of malaria parasites prevalent in malaria endemic countries worldwide. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and evaluated in comparison with thick blood smear microscopy, an antigen-based rapid diagn...
Human African trypanosomiasis (HAT) is a vector-borne parasitic zoonotic disease. The disease caused by Trypanosoma brucei gambiense is the most prevalent in Africa. Early diagnosis is hampered by lack of sensitive diagnostic techniques. This study explored the potential of loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) in the detection of T. b. gambiense infe...
La rápida propagación mundial de un nuevo coronavirus denominado SARS-CoV-2, detectado en la cuidad Wuhan, China, hace necesario del conocimiento e implementación métodos diagnóstico confiables para detectar y tratar adecuadamente a los pacientes. Para ello, más utilizados el son: técnica inmunocromatografía (IC), cual enmarca tanto las pruebas detectoras anticuerpos como prueba detección antíg...
Loop-mediated amplification (LAMP) amplifies specific nucleotide sequences but unlike PCR, it runs at a single temperature and positive reactions can be visualized with the naked eye so fragile instrumentation is not required. We have developed a one step, single-tube, accelerated, FMDVspecific RT-LAMP assay for the rapid detection of all seven serotypes of the virus, using primers designed aga...
background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...
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