Following the widely spread EPR spin-label applications for biosystem characterization, a novel approach is proposed for EPR-based characterization of biosystem complexity. Hereto a computational method based on a hybrid evolutionary optimization (HEO) is introduced. The enormous volume of information obtained from multiple HEO runs is reduced with a novel so-called GHOST condensation method fo...

A fundamental question of biology is how nucleic acids first assembled and then were incorporated into the earliest forms of cellular life 4 billion years ago. The polymerization of nucleotides is a condensation reaction in which phosphodiester bonds are formed. This reaction cannot occur in aqueous solutions, but guided polymerization in an anhydrous lipid environment could promote a non-enzym...

Adaptation and placement of alpha-phase gutta-percha delivered with a plastic core-carrier, Thermafil, was compared to the lateral condensation of gutta-percha in a specific tooth model. Fifty-one mandibular molar roots with separate canals, patent canal orifices and curvatures greater than 15 degrees were cleaned and shaped with K-files and 2.5% sodium hypochlorite to a size 30 at the apex, an...

This in vitro study aims to evaluate the sealing ability of gutta percha root filling used with mineral trioxide aggregate [MTA], and zinc-oxide eugenol as sealer. Thirty freshly extracted single-roote teeth were selected, decoronated and then instrumented. Samples were randomly divided into five experimental groups. In group 1, root canals were filled using single cone gutta percha technique, ...

OBJECTIVE To determine the percent gutta-percha-filled area in the apical third of root canals after filling with 2 different root filling techniques. MATERIALS AND METHODS Thirty extracted human lower premolars were instrumented with ProTaper rotary files (Dentsply Maillefer) and then randomly divided into 2 groups of 15 teeth each. The first group was filled using the single-cone technique ...

In endodontics, a laser can be used as a heat source and gutta-percha can be softened by a laser and used in the root canals. The System-B endodontic heat source unit was designed for the obturation of the root canal system with a single continuous wave of thermoplasticized gutta-percha. The aim of this study was to compare the apical leakage of lateral condensation; Nd: YAG laser-softened gutt...

This study aimed at providing a gauge device (Ekontak et al Gauge K-Device) in order to analyze the forces applied to teeth and periodontal tissues during dental practices in vitro. This force gauge device can be used in the investigation of the possible defect generation to tooth structures when overloaded forces are applied during dental procedures in vitro. Ekontak et al Gauge K-Device consi...

The aim of this in vitro study was to compare the sealing quality of three root canal filling materials after four years of ageing. Obturation with the non-instrumentation technique (NIT) was compared with cold lateral condensation of gutta-percha. Seventy-six canals were endodontically treated with traditional hand instrumentation and cold lateral condensation (control) whereas 77 canals were ...

STATEMENT OF PROBLEM Studies reported that nearly 60% of endodontic failures have been attributed to inadequate obturation of the root canal system. Thus, complete obturation of the root canal system and proper apical seal are essential elements in the long-term success of root canal treatment. PURPOSE This study aimed to compare the apical seal of lateral condensation technique in the room a...