Polyethyleneimine (PEI)-alginate (Alg) nanoparticle (NP) is a safe and effective vector for delivery of siRNA or DNA. Recent studies suggest that autophagy is related to cytotoxicity of PEI NPs. However, contribution of autophagy to degradation of PEI-Alg NPs remains unknown. CD34+VEGFR-3+ endothelial progenitor cells isolated from rat bone marrow were treated with 25 kDa branched PEI modified ...

Autophagy is an important process in the pathogenesis of atherosclerosis. C1q/tumor necrosis factor-related protein 9 (CTRP9) closest adiponectin paralog. CTRP9 has anti-aging and anti-atherogenic effects, but its roles autophagy endothelial senescence are currently unknown. This study aimed to evaluate whether prevents palmitic acid (PA)-induced by promoting autophagy. After no treatment or pr...

Grinding aids are commonly used in cement manufacture to reduce electrostatic forces between powder particles and agglomeration. Alkanolamines known also influence the hydration of aluminate phases cement. This study assessed effect TEA, TIPA DEIPA addition on LC3 systems. It was observed that these molecules have an enhancing reaction LC3. They promote ferrite C3A, lead higher amounts hemicarb...

It has been reported that cornin may reduce neuronal death during cerebral ischemia; however, little is known about the molecular mechanism of the role of corninin autophagy in SH‑SY5Y neuronal cells. In the present study, oxygen‑glucose deprivation (OGD)‑treated cells were used as a cerebral ischemia model in vitro. The results demonstrated that cornin was able to reduce neuronal cell loss, in...

BACKGROUND Nasu-Hakola disease (NHD) is a rare autosomal recessive disorder characterized by sclerosing leukoencephalopathy and multifocal bone cysts, caused by a loss-of-function mutation of either DAP12 or TREM2. TREM2 and DAP12 constitute a receptor/adaptor signaling complex expressed exclusively on osteoclasts, dendritic cells, macrophages, and microglia. Neuropathologically, NHD exhibits p...

Autophagy is a cellular degrading process that promotes tumor cell survival or cell death in cancer, depending on the progress of oncogenesis. Protein light chain 3 (LC3) and p62/SQSTM1 (p62) are associated with autophagosomal membranes that engulf cytoplasmic content for subsequent degradation. We studied LC3 and p62 expression using immunohistochemistry in a large cohort of 466 stage I/II non...

Autophagy is a cellular self-eating process that plays an important role in neuroprotection as well as neuronal injury and death. The detailed pathway of autophagy in these two opposing functions remains to be elucidated. Neurons are highly specialized, postmitotic cells that are typically composed of a soma (cell body), a dendritic tree, and an axon. Here, we describe methods for studying auto...

We examined selective autophagy against ubiquitinated protein aggregates in sporadic inclusion body myositis (s-IBM) patients. The form of autophagy requires phosphorylation of serine 403 in p62/SQSTM1 to bind to Lys63-linked ubiquitin and the binding of the p62-ubiquitinated protein conjugates to LC3. In muscle biopsy specimens from 16 s-IBM patients, we compared the distribution of p62 (aa120...

s were prepared by the authors and printed as submitted. T H U R S D A Y, JU N E 2, 2016 was carried out in the third group (DM-EX group) for four weeks. The animals were sacrificed; the blood were collected; the blood glucose was measured; the autophagy relationship proteins (i.e., Atg5, LC3-I, LC3-II, Atg12) and apoptosis proteins (i.e., Beclin1, caspase3, cleaved caspase3, Bcl2, P-Bcl2) were...

BACKGROUND Recent studies have suggested that autophagy is utilized by cells as a protective mechanism against Listeria monocytogenes infection. METHODOLOGY/PRINCIPAL FINDINGS However we find autophagy has no measurable role in vacuolar escape and intracellular growth in primary cultured bone marrow derived macrophages (BMDMs) deficient for autophagy (atg5-/-). Nevertheless, we provide eviden...