Sheep pituitary cells prelabelled with radioactive [35S] sulfate (35SO4(2-)) were incubated with different concentrations of LH-RH and the release of LH (lutropin) into the medium was monitored in terms of immunoprecipitable [35S] sulfated LH radioactivity and estimation of LH in the same sample by radioimmunoassay. A dose dependent response was obtained with a maximum of a 16 fold increase in ...

Lysyl hydroxylase (LH) isoform 3 is a post-translational enzyme possessing LH, collagen galactosyltransferase (GT) and glucosyltransferase (GGT) activities. We have demonstrated that LH3 is found not only intracellularly, but also on the cell surface and in the extracellular space, suggesting additional functions for LH3. Here we show that the targeted disruption of LH3 by siRNA causes a marked...

The plasma metabolic clearance of biologically active luteinizing hormone (bioactive LH) was studied using the rat interstitial cell testosterone (RICT) bioassay in six hypogonadotropic men after single bolus injection of highly purified human LH and during continuous steady-state infusions of three graded doses of LH. The LH bolus disappearance curves provided estimates of metabolic clearance ...

Profiles of LH and FSH levels and pituitary LH responses to exogenous luteinizing hormone releasing hormone (LHRH) have been characterized in rams, castrated rams (wethers), and wethers implanted with testosterone. Rams were castrated when adult, and, at the time of castration, two groups of wethers were implanted with either four or eight testosterone capsules. Rams showed random pulses of LH ...

There is a need to develop alternative disinfectants that differ from conventional antibiotics address antibacterial resistance, along with specialized materials for biomedical applications. Herein, we report on the synthesis of zinc sulfide (ZnS) capped chitosan (CS) produce CS-ZnS nanocomposites (NCs), which were assayed activity in liquid handwash formulations. The NCs prepared using bottom-...

Introduction The addition of antagonists is mainly based on estrogen level and follicle size, while LH has not received sufficient attention.In this study, Level the antagonist administration day was used as main research objective to explore its relationship with laboratory indicators pregnancy outcomes. Methods Analysis We enrolled 854 patients normal ovarian function undergoing in-vitro fert...

but the active/latent isoform proportions were similar (Fig. 1A). Platelet aggregation during clotting (6) may have caused these differences. MMP expression was lower in K 2 E plasma than in LH plasma [14 (3) vs 25 (4) ␮g/L; P Ͻ0.05; Fig. 1A, lane 4 vs lane 5]. The concentrations of MMP-9 forms decreased significantly with increasing amounts of K 2 E during PB collection, whereas MMP-2 was incr...

It is known that the early environment affects the mental development of rodent and human offspring. However, it is not known specifically whether a postpartum depressive state influences the depressive state in offspring. Using learned helplessness (LH) in rats as an animal model of depression, we examined the influence of maternal postpartum LH on responses to the LH test of offspring. Dam ra...

Gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have been implicated as probable risk factors in epithelial ovarian carcinomas, most of which are derived from ovarian surface epithelium (OSE). Since epidermal growth factor (EGF) increases the growth of ovarian surface epithelial cells, we determined the effect of gonadotropins on the expression of epidermal growth...

To investigate a mode and a site of action of inhibin on FSH and LH secretion, in vivo and in vitro effects of porcine follicular fluid (PFF) on FSH and LH secretion were studied in male rats. PFF administered i.v. suppressed basal FSH levels for 1-8 hr, but did not alter LH levels. On the contrary, PFF given either 1 or 6 hr in advance, suppressed LH-RH stimulated LH responses as well as LH-RH...