Previous works have demonstrated that ligninolytic enzymes mediated effective degradation of lignin wastes. The degrading ability greatly relied on the interactions of ligninolytic enzymes with lignin. Ligninolytic enzymes mainly contain laccase (Lac), lignin peroxidase (LiP) and manganese peroxidase (MnP). In the present study, the binding modes of lignin to Lac, LiP and MnP were systematicall...

Hydroxyphenyl (H-type) and guaiacyl (G-type) lignin model polymers composed of the β–O–4 structure without –hydroxymethyl groups were synthesized. The chemical structures of the Hand G-type lignin models were characterized by Hand C-NMR, as well as MALDI-TOF/MS. The pyrolysis response was analyzed by means of TG-DTG, Py-GC/MS, and a tube furnace technique. H-, C-NMR, and MALDI-TOF/MS showed th...

In order to obtain the adhesion force of fiber in a paper sheet easily, the relationships between internal bonding strength (IBS) and surface lignin content of masson pine CTMP treated with peracetic acid (PAA) have been investigated with XPS technique, and the surface morphology of fibers was also imaged by AFM. The results showed that the extent of lignin covered on the fiber surface was two ...

The electrostatic and hydrophobic interactions between lignin and enzymes play essential roles in the effective enzymatic hydrolysis of lignocellulosic biomass. The negative effect of lignin has been closely associated with pretreatment efficiency and substrate hydrolysability. However, remarkably we have found contrasting effects of hardwood organosolv lignin (EOL-SG) and softwood organosolv l...

of chemical bonds but can also catalyze the radical-based re-polymerization of lignin fragments. In contrast, nonradical lignolytic enzymes selectively cleave chemical bonds in lignin and lignin model compounds and, thus, show promise for use in the preparation of high valueadded chemicals. In this mini-review, recent developments on non-radical lignolytic enzymes are discussed. These include r...

Background: The first genome of a selective lignin-degrader is available. Results: Its screening shows 26 peroxidase genes, and five were heterologously expressed and catalytic properties investigated. Conclusion: Two new peroxidases oxidize simple and dimeric lignin models and efficiently depolymerize lignin. Significance: Although lignin peroxidase and versatile peroxidase had not been report...

Understanding the mechanisms underlying plant biomass recalcitrance at the molecular level can only be achieved by accurate analyses of both the content and structural features of the molecules involved. Current quantification of lignin is, however, majorly based on unspecific gravimetric analysis after sulfuric acid hydrolysis. Hence, our research aimed at specific lignin quantification with c...

BACKGROUND In higher plant cells, lignin provides necessary physical support for plant growth and resistance to attack by microorganisms. For the same reason, lignin is considered to be a major impediment to the process of deconstructing biomass to simple sugars by hydrolytic enzymes. The in situ variation of lignin in plant cell walls is important for better understanding of the roles lignin p...

Breeding soybean for high seed quality is an important approach for developing cultivars for tropical regions, and the lignin content in the seed coat is one of the screening parameters for this trait. Considering that many breeding lines are evaluated in each growing season using the presently recommended method for lignin determination, a long period is required for the evaluation of the whol...

Bedouin goats were fed on wheat straw as a single-component diet under two watering regimens, drinking once daily or once every 4 d, in order to clarify whether lignin-degradation products were absorbed, metabolized and excreted in urine. Acid-soluble lignin accounted for 220 g/kg total lignin, its digestibility was the highest (0.87) and was unaffected by water deprivation. Acid-insoluble lign...