COMPARATIVE EVALUATION OF SPECIFIC ACTIVITY OF CRUDE AND PARTIALLY PURIFIED LIPASE PREPARATION FROM LIPOLYTIC BACTERIA Dr. Kanika Sharma and Madhu Rathore* Address for Correspondence Microbial Research Laboratory, Department of Botany, University College of Science, M.L.S. University Udaipur, Rajasthan – 313001, India E-mail: [email protected] ABSTRACT Most of the medical and commercial ...

The effect of bioaugmentation with an anaerobic lipolytic bacterial strain on the anaerobic digestion of restaurant lipid-rich waste was studied in batch experiments with a model waste containing 10% lipids (triolein) under two sets of experimental conditions: (A) methanogenic conditions, and (B) initially acidogenic conditions in the presence of only the lipolytic strain biomass (4 days), foll...

The in vitro lipolytic response to norepinephrine by rat adipocytes from epididymal, subcutaneous, perirenal, mesenteric, and omental tissue sites was studied in control and hypotensive animals. Lipolysis per millimole of triglyceride was found to be three to four times higher in mesenteric and omental fat cells than in adipocytes of the other sites sampled. The high lipolytic activity of mesen...

Serpa is an ovine raw milk cheese widely appreciated by the consumers. However, products may be seen with reservations in terms of safety or technological defects. To overcome that, addition autochthonous starter culture ensure cheesemaking process optimization and microbiota dominance. In this work, protective performance eleven lactic acid bacteria strains, isolated from Protected Designation...

In a bioprospecting effort towards novel thermostable lipases, we assessed the lipolytic profile of 101 bacterial strains isolated from the volcanic area of Santorini, Aegean Sea, Greece. Screening of lipase activity was performed both in agar plates and liquid cultures using olive oil as carbon source. Significant differences were observed between the two screening methods with no clear correl...

Eurycomanone (1) and 13β,21-epoxyeurycomanone (2) were isolated from Eurycoma longifolia for studies of lipolytic activity. Compound 1 enhanced lipolysis in adipocytes with an EC50 of 14.6μM, while its epoxy derivate, compound 2, had a stronger activity with an EC50 of 8.6μM. Based on molecular mechanistic study using several specific inhibitors to lipolytic signaling pathways, it was found tha...

Studying lipase in germinating sunflower seedlings, we looked for an activator of the lipolytic activity. In the presence of 1.25 mM ATP, the enzyme activity increased 2-fold. Lipid-body lipase solubilization was realized using two detergents: Tween 80 and CHAPS. Lipolytic activity was increased 10-fold in the presence of 2% (w/v) CHAPS, showing the probable 'complexity' of the enzyme. Looking ...

Yarrowia lipolytica is a very important yeast because many strains from this yeast are able to produce the extracelular lipases. Cold active lipase is one of the important and widely used enzymes whose spectrum of applications has widened in many industries such as in detergent formulations, food industry, leather processing, environmental bioremediations, and fine chemical synthesis as well as...

BACKGROUND Many waste streams have a relatively high vegetable oil content, which is a potential resource that should be recovered. Microbial storage compound production for the recovery of lipids from lipid-water emulsions with open (unsterilized) microbial cultures was investigated in a sequencing batch reactor using a diluted vegetable oil emulsion as model substrate. RESULTS After feeding...

Artisanal cheeses are prepared using traditional methods with territorial, regional and cultural linkages. In Brazil, there is a great diversity of artisanal (BAC), which have historical, socioeconomic importance. The the BAC between producing regions due to different compositions raw milk, steps involved in process maturation time. crucial step for cheese differentiation non-addition starter c...