The fluorescent phospholipid dansyl phosphatidylethanolamine (DPE) (dansyl, 5-dimethylaminonaphthalene-1-sulfonyl) was incorporated into very low density lipoproteins (VLDL) to form DPE-VLDL. The addition of milk lipoprotein lipase to DPE-VLDL in the presence of albumin resulted in a greater than %fold fluorescence increase and a 20 nm blue shift in the wavelength of the emission maxima of t...

BACKGROUND Patients with lipoprotein lipase deficiency usually present with chylomicronemia in childhood. The syndrome has been considered nonatherogenic primarily because of the low levels of low-density lipoprotein (LDL) cholesterol. We prospectively evaluated patients with lipoprotein lipase deficiency for atherosclerosis. METHODS Evidence of carotid, peripheral, and coronary atheroscleros...

The lipoprotein lipase activity per adipocyte was increased in the genetically obese rat (fa/fa). However, there was no difference between obese and lean animals when the enzyme activities were related to adipocyte surface area. The possible implications of the findings are discussed.

Dietary triglycerides are hydrolyzed in the small intestine principally by pancreatic lipase. Following uptake by enterocytes and secretion as chylomicrons, dietary lipids are cleared from the bloodstream via lipoprotein lipase. Whereas lipoprotein lipase is inhibited by several proteins including Angiopoietin-like 4 (Angptl4), no endogenous regulator of pancreatic lipase has yet been identifie...

In recent publications (l-3), the clearing factor of postheparin plasma has been identified with a lipoprotein lipase obtained from rat, rabbit, and calf heart and rat and rabbit adipose tissue. This enzyme catalyzes the hydrolysis of the triglyceride moiety of chylomicrons and low density @-lipoproteins. The hydrolysis of simple triglyceride emulsions is also catalyzed by lipoprotein lipase bu...

Macromolecular heparin from rat skin shows poor lipoprotein lipase-releasing activity in vivo and is a potent inhibitor of rat-heart lipoprotein lipase in vitro. Rat-skin heparin is depolymerized by incubation with the 100,000 x g supernatant from a sonicated homogenate of rat small intestine. The depolymerized products, fractionated by gel filtration, range from inhibitors to activators of lip...

Lipoprotein lipase enhances binding at 4°C of human plasma lipoproteins (chylomicrons, VLDL, intermediate density lipoprotein, LDL, and HDL3) to cultured fibroblasts and hepG-2 cells and to extracellular matrix. Heparinase treatment of cells and matrix reduces the lipoprotein lipase enhanced binding by 90-95%. Lipoprotein lipase causes only a minimal effect on the binding of lipoproteins to hep...