lipoxygenase(lox) catalyzes irreversible transfer of oxygen molecule to arachidonic and linoleic acid to produce 13 hydroproxy octadecadienoic acid. recent studies showed the involvement of lipoxygenase products, leukotrienes, in inflammations and lipoxygenase pathways acts as mediators of early inflammatory events in atherosclerosis. the aim of the present study was purification and characteri...

Using p-aminothiophenol (PATP) molecules on a gold substrate and high-vacuum tip-enhanced Raman spectroscopy (HV-TERS), we show that the vibrational spectra of these molecules are distinctly different from those in typical surface-enhanced Raman spectroscopy. Detailed first-principles calculations help to assign the Raman peaks in the TERS measurements as Raman-active and IR-active vibrational ...

The title Mn(II) complex, [Mn(C(9)H(10)NO(2))(2)(C(6)H(6)N(2)O)(H(2)O)(2)], contains two 4-(dimethylamino)benzoate (DMAB) anions, one isonicotinamide (INA) ligand and two coordinated water mol-ecules. One of the DMAB anions acts as a bidentate ligand, while the other is monodentate. The four O atoms in the equatorial plane around the Mn atom form a highly distorted square-planar arrangement, wh...

OBJECTIVE Aortic 15-lipoxygenase (15-LO) metabolizes arachidonic acid (AA) to 15-hydroperoxyeicosatetraenoic acid, which is then converted to the vasodilators 15-hydroxy-11,12-epoxyeicosatrienoic acid and 11,12,15-trihydroxyeicosatrienoic acid. These metabolites contribute to endothelium-dependent relaxations of rabbit aorta to AA and acetylcholine. We investigated the identity of rabbit aortic...

7 epoxide-containing compounds: allylbenzene oxide, styrene oxide, trans-beta-methylstyrene oxide, 4-chlorophenyl glycidyl ether, vinylcyclohexene dioxide, octene dioxide and hexene dioxide were evaluated for mutagenic activity in 4 histidine-requiring strains of Salmonella typhimurium, namely: TA1535, TA100, TA1537 and TA98. These epoxides, except trans-beta-methylstyrene oxide, were mutagenic...

Soybean lipoxygenases (soybean lipoxygenase-1) has been a well-studied enzyme because it is considered as model for mammalian lipoxygenases. In general, the soybean lipoxygenase-1 used in test of inhibitory activities various compounds. The present study provides bioinformatics approach comparison databases. Their physical and chemical parameters such molecular weight, theoretical pI, amino aci...

Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at lambdamax 628 nm. The second method describes the ...

Soybean lipoxygenase-1 (SLO-1) is a paradigmatic enzyme system for studying the contribution of hydrogen tunneling to enzymatic proton-coupled electron transfer processes. In this study, the impact of pairs of double mutants on the properties of SLO-1 is presented. Steady-state rates and their deuterium kinetic isotope effects (KIEs) have been measured for the bimolecular reaction of enzyme wit...

Kinetic parameters for the thermal inactivation of several enzymes in carrot and potato homogenates have been determined. In carrots the most heat-resistant activity was polygalacturonase, followed by peroxidase and pectinmethylesterase. In potatoes peroxidase was the most resistant, followed by pectin methylesterase, polyphenol oxidase, and lipoxygenase. There were several notable similarities...