Human peripheral blood monocytes (HPBM) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. After a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. Paralleli...

Bcl11a is a transcription factor known to regulate lymphoid and erythroid development. Recent bioinformatic analysis of global gene expression patterns has suggested a role for Bcl11a in the development of dendritic cell (DC) lineages. We tested this hypothesis by analyzing the development of DC and other lineages in Bcl11a (-/-) mice. We found that Bcl11a was required for expression of IL-7 re...

Nonalcoholic fatty liver disease (NAFLD) is a spectrum that spans simple steatosis, fibrosis, and ultimately cirrhosis, leading cause of chronic globally. The severe variant NAFLD, non-alcoholic steatohepatitis (NASH), characterized by triglyceride accumulation within hepatocytes the subsequent inflammatory pathway activation, progressing to cirrhosis in 10%-20% patients. NASH known major risk ...

Despite considerable progress in the design of multifunctionalized nanoparticles (NPs) that selectively target specific cell types, their systemic application often results unwanted liver accumulation. The exact mechanisms for this general observation are still unclear. Here we asked whether number cell-targeting antibodies per NP determines extent accumulation and also addressed by which antib...

Human CD34+CD38- hematopoietic precursor cells from fetal liver are able to develop into T, NK, and dendritic cells in a hybrid human/mouse fetal thymic organ culture (FTOC). In this report, we pay particular attention to the early events in differentiation of these precursor cells. We show that the CD34+CD38- precursor cells, which are CD4-CD7-cyCD3-HLA-DR-/++ (cy, cytoplasmatic), differentiat...

hla-g is normally expressed on human trophoblast cells. it is a non-classical mhc molecule class i b. the role of hla-g in diabetic type 1 is not known. we investigated the role of ifn-β in induction hla-g expression on the monocyte derived dendritic cells (dc) in diabetes type 1. treatment of dendritic cell with ifn-β in vitro from diabetic patients (n=20) and normal subjects (n=20) resulted t...

This research evaluates the effects of laminarin on the maturation of dendritic cells and on the in vivo activation of anti-cancer immunity. In vivo treatment of C56BL/6 mice with laminarin increased the expression levels of co-stimulatory molecules and the production of pro-inflammatory cytokines in spleen dendritic cells. Laminarin enhanced ovalbumin antigen presentation in spleen dendritic c...

Objective: The hippocampal formation, particularly the dentate gyrus (DG), shows age-related morphological changes that could cause memory decline. It is indicated that Boswellia resins attenuates memory deficits and the major component of Boswellia serrata (Bs) gum resin, beta boswellic acid increased neurite outgrowth and branching in hippocampal neurons. This study was designed to investigat...

Cirrhosis is among the leading causes of death worldwide, making up 3.5% global deaths. It characterized by replacement liver parenchyma with fibrotic tissue and progressively leads to complete failure organ. classified into 2 types – compensated decompensated based on complications such as esophageal varices, hepatic encephalopathy, asterixis, ascites. The main contributors disease include inf...