Agarose electrophoresis (Isopal, Beckman) and an immunoradiometric assay (IRMA) involving specific monoclonal antibodies (Ostase, Hybritech), two methods for the quantification of serum bone alkaline phosphatase (ALP, EC 3.1.3.1), a marker of osteoblastic activity, were compared in 293 patients: 79 with end-stage renal failure treated with hemodialysis and 214 with malignant disease. Overall co...

BACKGROUND Alkaline phosphatase (ALP) has been suggested to be associated with cardiovascular disease (CVD) risk, however, important aspects of the association, such as shape and independence from established risk factors, have yet to be characterized in detail. We assessed the association of ALP with CVD risk and determined its utility for CVD risk prediction. METHODS Alkaline phosphatase ac...

BACKGROUND Breast cancer is the commonest malignancy of women in Nigeria. Change in serum levels of some biochemical parameters could assist diagnosis and follow-up of breast cancer. OBJECTIVE To determine serum levels of calcium, inorganic phosphates, alkaline phosphatase (ALP) and acid phosphatase (ACP) activities in patients with breast cancer, and change in the serum levels over time. M...

The D 9 8 / A H 2 cell line (a subline of HeLa) expresses a form of alkaline phosphatase (ALP) which closely resembles the adult and fetal intestinal forms of ALP. To character ize this ectopic form of ALP, four monocIonal antibodies were raised against D 9 8 / A H 2 ALP, and their binding with ALPs from D 9 8 / AH-2 cells, placenta, fetal intestine (meconium), adult intestine, and liver was co...

Localization of alkaline phosphatase (ALP) and cathepsin D (CAPD) in primary cultures of fetal rat hepatocytes was examined using double immunofluorescent staining in order to investigate the relationship between lysosome movement and the fate of ALP during cell restoration after microtubule disruption by colchicine. At 3 hr and 24 hr after colchicine treatment, numerous coarse dots containing ...

the objective of this study was to investigate aspartate-amino-transferase (ast), γ-glutamyl-transferase(ggt), alkaline phosphatase (alp), lactate-dehydrogenase (ldh) and acid phosphatase (acp) activities andsemen parameters (volume, ph, concentration, total sperm number (tsn), progressive motility, dead sperm,total morphological defect (tmd) and hypo-osmotic swelling test (host)) in seminal pl...

The D98/AH-2 cell line (a subline of HeLa) expresses a form of alkaline phosphatase (ALP) which closely resembles the adult and fetal intestinal forms of ALP. To characterize this ectopic form of ALP, four monoclonal antibodies were raised against D98/AH-2 ALP, and their binding with ALPs from D98/AH-2 cells, placenta, fetal intestine (meconium), adult intestine, and liver was compared using an...

The D 9 8 / A H 2 cell line (a subline of HeLa) expresses a form of alkaline phosphatase (ALP) which closely resembles the adult and fetal intestinal forms of ALP. To character ize this ectopic form of ALP, four monocIonal antibodies were raised against D 9 8 / A H 2 ALP, and their binding with ALPs from D 9 8 / AH-2 cells, placenta, fetal intestine (meconium), adult intestine, and liver was co...

Alkaline phosphatase (ALP) enzyme is naturally present in raw milk which used as a prominent indicator to determine proper pasteurization milk. However, determination of ALP activity either time-consuming or required sophisticated instrument facilities that are uncommon India's rural dairy collection centers. Present test based on the reaction with p-nitrophenyl phosphate release p-nitrophenol ...