Plasmodium, Isospora, Toxoplasma, Babesia and Cryptosporidium are parasites which have a significant role in human health. The location in which the protozoon settles in the body, the way that it leaves the host, and the sample which is send from the clinic are the elements of the general methods used in the diagnosis of the ailments caused from protozoan. Classical methods are not adequate for...

Digital nucleic acid detection is rapidly becoming a popular technique for ultra-sensitive and quantitative detection of nucleic acid molecules in a wide range of biomedical studies. Digital polymerase chain reaction (PCR) remains the most popular way of conducting digital nucleic acid detection. However, due to the need for thermocycling, digital PCR is difficult to implement in a streamlined ...

As a result of the importance of quick diagnosis of Neurocryptococcosis in patients, generally, molecular techniques are preferred rather than traditional methods for their rapidity. In this research, we made an effort to design and optimize Loop mediated isothermal AMPlification (LAMP) technique using specific primers for ura5 as the target gene for detection of Cryptococcus neoformans and Cry...

The loop-mediated isothermal amplification (LAMP) assay indicates a potential and valuable means for genetically modified organism (GMO) detection especially for its rapidity, simplicity, and low cost. We developed and evaluated the specificity and sensitivity of the LAMP method for rapid detection of the genetically modified (GM) maize T25. A set of six specific primers was successfully design...

The most common direct detection methods are isolation or in-vitro cultivation, electron microscopy, immunofluorescence, immunohistochemistry, antigen enzyme-linked immunosorbent assay (antigen-ELISA), nucleic-acid hybridisation (NAH), macroand microarrays and the various techniques of nucleic acid amplification, such as the polymerase chain reaction (PCR) or the isothermal amplification method...

The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that ...

We have developed a novel method, termed loop-mediated isothermal amplification (LAMP), that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. An inner primer containing sequences of the sense and antisense ...

Influenza virus infections represent a worldwide public health and economic problem due to the significant morbidity and mortality caused by seasonal epidemics and pandemics. Sensitive and convenient methodologies for detection of influenza viruses are essential for further disease control. Loop-mediated isothermal amplification (LAMP) is the most commonly used method of nucleic acid isothermal...

Ratoon stunt, caused by the xylem-limited coryneform bacterium Leifsonia xyli subsp. xyli (Lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries. Based on loop-mediated isothermal amplification (LAMP), we developed a method for detecting Lxx. The major advantages of the LAMP method are visual judgment by color and time saving with only 60 min for identification of L...

In this study, we developed rapid and sensitive assays for the detection of Cladophialophora carrionii, a common agent of human chromoblastomycosis. The isothermal techniques evaluated were rolling-circle amplification (RCA), multiplex ligation-dependent probe amplification (MLPA), and loop-mediated isothermal amplification (LAMP). The probes for RCA and MLPA were designed with target sequences...