BACKGROUND At the time of the study, the HIV-treatment policy in South Africa included highly active antiretroviral therapy (HAART) regimens 1 (nucleotide reverse transcriptase inhibitors (NRTIs) only), and 2 (protease inhibitors (PI) and NRTIs). HAART is associated with the lipodystrophy syndrome, insulin resistance and reduced total adiponectin (TA) levels. The high molecular weight (HMW):TA ...

The localisation of a low-molecular-weight bronchial protease inhibitor (LMI) in human peripheral lung tissue was studied by an immunohistochemical method. This inhibitor was found exclusively in a part of the non-ciliated cell population of the bronchiolar epithelium. An inverse relationship was observed between the number of LMI-positive non-ciliated cells per millimetre of basement membrane ...

The study was carried out to identify muscle protein patterns and gut protease activity of rohu (Labeo rohita), catla (Catla catla), climbing perch (Anabas testudineus) spotted snakehead (Channa punctatus). Twelve percent Polyacryleamide substrate SDS-PAGE done visualize the enzyme after casein hydrolysis pattern species. Three alkaline proteases different molecular weight were observed in rohu...

Kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, Salinivibrio sp. strain MS-7, and maximum growth and protease activity was achieved after 48 hours at 30°C and 180 rpm. To determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and  maximum production of...

Isolates of atypical scrapie recently identified in sheep and goats in France were compared with Nor98 isolates reported in Norway. Western blot methods for characterization of the protease-resistant prion protein showed that all these isolates shared a unique biochemical signature: 5 groups of bands, including a characteristic band of apparent low molecular weight (11 kDa). This pattern could ...

Alkaline protease enzyme was produced under solid state fermentation by Fusarium solani. Various agro-wastes were evaluated to check their potential utilization in the solid state fermentation for protease production. In this connection, the results showed that rice straw was the best agro-waste. The best enzyme activity was achieved at 7th day of incubation, pH 8, temperature 45 °C, inoculum’s...

An intracellular serine protease, which is believed to initiate the degradation of several intracellular pyridoxal phosphate-dependent enzymes, was localized by immunofluorescence in atypical mast cells of the lamina propria and in intraepithelial cells of the rat small intestine. Some mucus-secreting goblet cells also contained the protease antigen. Atypical mast cells containing the enzyme we...

A total of 13 Photorhabdus luminescens strains were screened for proteolytic activity. The P. luminescens strain 0805-P5G had the highest activity on both skim milk and gelatin plates. The protease was purified to electrophoretical homogeneity by using a two-step column chromatographic procedure. It had a molecular weight of 51.8 kDa, as determined by MALDI-TOF mass spectrometry. The optimum pH...