A REPORT by a working party of the Public Health Laboratory Service on the laboratory diagnosis of tuberculosis, reported in the Monthly Bulletin of the Ministry of Health and Public Health Laboratory Service (August, 1952, Vol. 11, p. 187 onwards) and referred to in the Bulletin of Hygiene (September, 1953, 28, 748), lays out the best methods of homogenization of sputa prior to culture on Lowe...

Methods Sixty lymph node aspirates from suspected cases of tuberculous lymphadenitis were examined for Mycobacterium tuberculosis by Ziehl neelsen (ZN) staining, culture on a Lowenstein Jensen medium, and cytological examination for findings suggestive of tubercular lymphadenitis. DNA from all the samples was amplified by conventional PCR targeting 123 bp and 240 bp fragments of IS6110 and MPT6...

Low-risk human papillomavirus types 6 and 11 can manifest as giant condylomata acuminata (GCA) of Buschke-Lowenstein. Up to 50% of GCA can slowly progress over years to fungating, invasive tumors. The malignant potential is attributed to unique immune evading abilities of the human papillomavirus. A 42-year-old male presented with pain and foul-smelling discharge from his genital warts. The his...

AIM Buschke-Lowenstein disease or giant condyloma acuminatum represents a rare, sexually transmitted disorder, with a slow evolution and the tendency to infiltrate in the adjacent tissues; untreated, the outcome is unfavorable. The hallmark is the development of one or various prominent-sized vegetant tumors that usually ulcerate. MATERIAL AND METHODS The present article summarizes both the e...

Methods The clinical specimens collected from HIV seropositive AFB smear negative patients (Group A) with features of TB, AFB smear positive, HIV reactive (Group B) and AFB smear positive, HIV negative (Group C) were processed as per standard protocol. They were inoculated onto Lowenstein Jensen media and the isolates obtained were subjected to drug susceptibility test (DST) by proportion metho...

Direct culture on Lowenstein-Jensen slopes and on three media made selective for tubercle bacilli by the addition of four antibacterial agents was compared with guinea-pig inoculation on 490 tissue specimens. Tubercle bacilli were obtained from 15 specimens by culture and 14 by guinea-pig inoculation; only one specimen was positive by guinea-pig and not by culture. The most efficient culture me...

Tuberculous meningitis (TBM) is a common entity n children and also in adults with prediosposing factors such as AIDS, alcoholism, diabetes mellitus, malignancy and use of steroids. TBM often leads to considerable morbidity and mortality. We present here CSF culture positive cases of TBM reported between 2000 and 2004 from our institution. CSF specimens from patients suspected of TBM were proce...