In mammals, embryonic development are highly regulated morphogenetic processes that are tightly controlled by genetic elements. Failure of any one of these processes can result in embryonic malformation. The lysyl oxidase (LOX) family genes are closely related to human diseases. In this study, we investigated the essential role of lysyl oxidase-like 3 (LOXL3), a member of the LOX family, in emb...

LYSYL OXIDASE INTERACTS WITH HORMONE PLACENTAL LACTOGEN AND SYNERGISTICALLY PROMOTES BREAST EPTHELIAL CELL PROLIFERATION AND MIGRATION Noemi Polgar1, Ben Fogelgren1, 2, J. Michael Shipley3, Katalin Csiszar1 1 Cardiovascular Research Center, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA 96822 2 Department of Anatomy, Biochemistry and Physiology, John A. Burns School o...

The transcription factor Snail controls epithelial-mesenchymal transitions (EMT) by repressing E-cadherin expression and other epithelial genes. However, the mechanisms involved in the regulation of Snail function are not fully understood. Here we show that lysyl-oxidase-like 2 and 3 (LOXL2 and LOXL3), two members of the lysyl-oxidase gene family, interact and cooperate with Snail to downregula...

We thank M. Krall and M. C. Adamson for expert technical assistance. M. spretus (Spain) mice came from a laboratory colony derived from mice originally trapped in Spain and maintained under NCI contract NO1-CB2-5584, S.C., K.K., and R.M.F. are supported by NIH Grant RO1 CA37351. 15. Siegel, R. C., Chen, K. H., Greenspan, J. S., and Aguiar, J. M. (1978). Biochemical and immunochemical study of l...

Lysyl-oxidase-like 2 (LOXL2) is a member of the lysyl oxidase family that catalyzes the cross-linking of collagens or elastins in the extracellular matrix, thus regulating the tensile strength of tissues. However, many reports have suggested different intracellular roles for LOXL2, including the ability to regulate gene transcription and tumor progression. We previously reported that LOXL2 medi...

As a result of a chemical genetic screen for modulators of metalloprotease activity, we report that 2-mercaptopyridine-N-oxide induces a conspicuous undulating notochord defect in zebrafish embryos, a phenocopy of the leviathan mutant. The location of the chemically-induced wavy notochord correlated with the timing of application, thus defining a narrow chemical sensitivity window during segmen...

Lysyl oxidase purified from urea extracts of various connective tissues resolves into multiple catalytically functional species upon chromatography on DEAE-cellulose in 6 M urea. The four enzyme species of bovine aorta retain their original chromatographic behavior on DEAE with time of storage and after purification to homogeneity by gel exclusion chromatography. The peptide maps of each aortic...