We developed a chemically defined medium called milieu proche du lait (MPL), in which 22 Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus) strains exhibited growth rates ranging from 0.55 to 1 h(-1). MPL can also be used for cultivation of other lactobacilli and Streptococcus thermophilus. The growth characteristics of L. bulgaricus in MPL containing different carbon sources were dete...

In this paper we report experiments demonstrating the presence of N-linked oligosaccharide structures in Toxoplasma gondii tachyzoites, providing the first direct biochemical evidence that this sporozoan parasite is capable of synthesizing N-linked glycans. The tachyzoite surface glycoprotein gp23 was metabolically labelled with [3H]glucosamine and [3H]mannose. Gel-filtration chromatography on ...

Cell Tracking In article number 2207626, Jeff W.M. Bulte, Chao Wang, and Ali Shakeri-Zadeh review the occasional adverse effects encountered with magnetic labeling of cells for in vivo cell tracking using resonance imaging (MRI) particle imaging. Mesenchymal stem (MSCs) are abundant mannose residues on their membrane. Using mannose-sensitive chemical exchange saturation transfer MRI, MSCs is no...

mannose-binding lectin (mbl) is a protein of innate immune system that is involved in opsonization and complement activation. mbl deficiency is associated with predisposition to infectious diseases; however subnormal levels are also seen in healthy subjects. the aim of this study was to investigate the prevalence and clinical manifestation of mbl deficiency in patients with increased susceptibi...

In this report we present an initial determination of the biochemical defect present in a Chinese hamster ovary cell line selected for resistance to concanavalin A. Membranes of this mutant, B211, incorporated at least 10-fold less mannose from GDP-[14C]mannose into oligosaccharide-lipid than membranes of the wild type. In the presence of dolichol phosphate, membranes of the mutant and wild typ...

D2 is a membrane glycoprotein that is believed to function as a cell adhesion molecule (CAM) in neural cells. We have examined its biosynthesis in cultured fetal rat brain neurones. We found D2-CAM to be synthesized initially as two polypeptides: Mr 186,000 (A) and Mr 136,000 (B). With increasing chase times the Mr of both molecules increased to 187,000-201,000 (A) and 137,000-158,000 (B). Thes...

Ascorbic acid (AsA) or Vitamin C is an antioxidant molecule and plays important role in many biological processes plants. GDP-D-mannose pyrophosphorylase (GMP VTC1) catalyzes the synthesis of GDP-D-mannose, which a precursor for AsA production used cell wall polysaccharide glycoprotein synthesis. In rice, OsVTC1 gene consists three homologs, including OsVTC1-1, OsVTC1-3 OsVTC1-8. this study, we...

Residual streams from lignocellulosic processes contain sugar mixtures of glucose, xylose, and mannose. Here, the industrial workhorse Corynebacterium glutamicum was explored as a research platform for rational utilization multiple substrate. The endogenous manA gene overexpressed to enhance mannose utilization. overexpression xylA Xanthomonas campestris in combination with xylB enabled xylose ...

The cell surface glycopeptides from an epithelial cell line (CCL 239) derived from normal human colon were compared with those from three cell lines (HCT-8R, HCT-15, and CaCo-2) derived independently from human colonic adenocarcinomas. Cells were incubated with D-[2-3H]mannose or L-[5,6-3H]fucose for 24 h and treated with trypsin to release cell surface components which were then digested exhau...

Type I carbohydrate-deficient glycoprotein syndrome (CDGS) patients fail to add entire N-linked oligosaccharide chains to some serum glycoproteins. Here we show that four CDGS fibroblast cell lines have two related glycosylation abnormalities. First, they incorporate 3-10-fold less [3H] mannose into proteins, and, second, the size of the lipid-linked oligosaccharide precursor (LLO) is much smal...