The contribution of regulatory genes to fluoroquinolone resistance was studied with clinical Escherichia coli strains bearing mutations in gyrA and parC and with different levels of fluoroquinolone resistance. Expression of marA and soxS was evaluated by Northern blot analysis of isolates that demonstrated increased organic solvent tolerance, a phenotype that has been linked to overexpression o...

Paralogous transcriptional regulators MarA, Rob, and SoxS act individually and together to control expression of more than 80 Escherichia coli genes. Deletion of marA, rob, and soxS from an E. coli clinical isolate prevents persistence beyond 2 days postinfection in a mouse model of pyelonephritis. We used microarray analysis to identify 242 genes differentially expressed between the triple del...

Comparative reverse transcription-PCR in combination with denaturing high-pressure liquid chromatography analysis was used to determine the levels of expression of soxS, marA, acrF, acrB, and acrD in multiple-antibiotic-resistant (MAR) Salmonella enterica serovar Typhimurium isolates and mutants of S. enterica serovar Typhimurium SL1344 with defined deletions. Posttherapy MAR clinical isolates ...

Peanut allergy (PNA) is the major cause of fatal and near-fatal anaphylactic reactions to foods. Traditional immunotherapy using peanut (PN) protein is not an option for PNA therapy because of the high incidence of adverse reactions. We investigated the effects of s.c. injections of engineered (modified) recombinant PN proteins and heat-killed Listeria monocytogenes (HKLM) as an adjuvant on ana...

  Objective(s): The major antibiotic efflux pump of Esherichia coli is AcrAB-TolC. The first part of the pump, AcrAB, is encoded by acrAB operon. The expression of this operon can be kept elevated by overexpression of an activator, MarA following inactivation of MarR and AcrR repressors due to mutation in encoding genes, marR and acrR, respectively. The aims of this research were to us...

Current trends of housing development and rapid enrolment change are impacting students’ daily life such as their housing comfort, convenience and safety. This research is undertaken to study the challenges faced by the students’ well-being with regards to off-campus living environment. This study requires a key informant interview survey with samples taken from Division of Students Affairs, Un...

objective(s): the major antibiotic efflux pump of esherichia coli is acrab-tolc. the first part of the pump, acrab, is encoded by acrab operon. the expression of this operon can be kept elevated by overexpression of an activator, mara following inactivation of marr and acrr repressors due to mutation in encoding genes, marr and acrr, respectively. the aims of this research were to use e. coli m...

Multiagent Resource Allocation (MARA) distributes a set of resources among a set of intelligent agents in order to respect the preferences of the agents and to maximize some measure of global utility, which may include minimizing total costs or maximizing total return. We are interested in MARA solutions that provide optimal or close-to-optimal allocation of resources in terms of maximizing a g...