OBJECTIVES The objective of this study was to assess the presence of mcr-1 in Shigella sonnei isolated in Vietnam. METHODS WGS data were analysed for the presence of the mcr-1 gene sequence. The association of mcr-1 with a plasmid was assessed by PCR and by conjugation. RESULTS Through genome sequencing we identified a plasmid-associated inactive form of mcr-1 in a 2008 Vietnamese isolate o...

The emergence of the plasmid-mediated mcr colistin resistance gene in the community poses a potential threat for treatment of patients, especially when hospitalized. The aim of this study was to determine the prevalence of all currently known mcr mediated colistin resistance gene in fecal samples of patients attending a tertiary care hospital. From November 2014 until July 2015, fecal samples o...

Mechanochemically responsive (MCR) polymers have been designed to possess unconventional properties such as changing colors, self-healing, and releasing catalysts under deformation. These properties of MCR polymers stem from a class of molecules, referred to as mechanophores, whose chemical reactions can be controlled by mechanical forces. Although extensive studies have been devoted to the syn...

The free chromophores (chr) extracted from macromomycin (MCR) and auromomycin (AUR) showed different IR spectra in KBr tablets and different CD spectra in ethanol or methanol solution. Chr-MCR contained a greater amount of alkyl moieties than chr-AUR as shown by much stronger IR absorbance at 2960 cm-1. The IR spectra of MCR, AUR and their apo-proteins (apo) in D2O showed that major parts of th...

surface. MCR is unique in that it apparently inhibits DNA synthesis while binding to the plasma membrane (12, 13). This mechanism of action requires that the binding of MCR to the cell surface freezes on modifies the moleof the mom bnane in mediating cellular metabolism. Kunimotoetal. (13) hypothesize that the interaction between MCR and tumor cells is analogous to the interactions between bact...

Simultaneous spectrophotometric analysis of a multi-component dosage form of olmesartan, amlodipine and hydrochlorothiazide used for the treatment of hypertension has been carried out using various chemometric methods. Multivariate calibration methods include classical least squares (CLS) executed by net analyte processing (NAP-CLS), orthogonal signal correction (OSC-CLS) and direct orthogonal ...

The Multilingual Central Repository (MCR) is based on the design of the EuroWordNet database. The MCR holds WordNets in various languages (English, Spanish, Italian, Catalan and Basque), which are interconnected via an Inter-LingualIndex (ILI). In addition, the MCR holds a number of ontologies and domain labels related to all concepts. This paper describes the integration and evaluation of the ...

The aim of the study was to develop a multiplex assay for rapid detection of mcr-1, mcr-2, and mcr-3, a group of genes of conferring resistance to colistin mediated by plasmid in Enterobacteriaceae. A SYBR Green based real-time PCR assay has been designed to detect the mcr genes, and applied to cultured bacteria, feces and soil samples. All three mcr genes could be detected with a lower limit o...

The dimerization constants of Safranine T have been determined by studying the dependence of its absorption spectra on the temperature in the range 30–70 ◦C at different total concentrations of Safranine T (1.03×10−5, 1.44×10−5 and 1.73×10−5 M). The monomer–dimer equilibrium of Safranine T has been determined by applying MCR-ALS method on the absorpti...

BACKGROUND Human fecal carriage of Enterobacteriaceae possessing mobilized colistin resistance genes (mcr-1 and mcr-2) remains obscure in Hong Kong. As part of routine surveillance on emerging antibiotic resistance, we conducted a prospective study on this topic in a regional hospital in Hong Kong. METHODS From October 31 to November 25, 2016, all fecal specimens submitted for routine analysi...