background and aims: influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. a variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. methods: nasal and pharyngeal samples taken from patients were inoculated into madin-darby canine kidney (mock) cells and embryonated chicken eggs (eces). the culture media was as...

several transporters appear to be important in transporting various drugs. many patients, who receive morphine as analgesic medication, also receive other medications with potency of changing morphine transport by affecting p-glycoprotein (p-gp) and oatp2 transport system. this could influence morphine pharmacokinetics and pharmacodynamics. the aim of present study was to elucidate the transpor...

The importance of virologically documented infections in influenza surveillance is well recognized and has been reaffirmed in recent reviews. The large number of specimens tested in surveillance make efficiency and low cost of virologic methods important. Based on observations made by others and our work with reisolation of stored specimens we have used the continuous line tissue cultures MDCK ...

Tight junctions, which locate at the apicolateral borders of epithelial cells to seal intercellular space, are previously proposed connect circumferential actomyosin belt, whose contractility exerting line tensions affects paracellular transport by opening pores tight junctions. Although junctions known display tortuous morphology, tension leads straight tight-junction morphology as energy mini...

Lactic acid bacteria are one of the potential natural remedies used worldwide, commonly known as probiotics. Here, aim this research investigation was to isolate a probiotic Lactobacilli strain, YLM015, from popular Korean fermented vegetable “Kimchi” and evaluate its anti-viral against influenza virus A (IFVA) H1N1 using MDCK cell line in vitro, embryonated eggs ovo. The YML015 strain selected...

Hepatocyte growth factor (HGF) elicits a broad spectrum of biological activities, including epithelial cell dedifferentiation. One of the most widely used and best-studied polarized epithelial cell lines is the Madin-Darby canine kidney (MDCK) cell line. Here, we describe and validate the early response of polarized monolayers of MDCK cells stimulated with recombinant HGF using a novel canine D...

Liver X receptors (LXRs) are transcription factors belonging to the nuclear receptor super family, which act as regulators of lipid and glucose metabolism. However, LXRs have been shown to regulate the function of transporters in the kidney, including the Na-Pi cotransporter, organic anion transporter, and epithelial Na(+) channel. In this report, we demonstrated the ability of LXR ligands, bot...

The Drosophila tumor suppressor protein lethal (2) giant larvae [l(2)gl] is involved in the establishment of epithelial cell polarity during development. Recently, a yeast homolog of the protein has been shown to interact with components of the post-Golgi exocytic machinery and to regulate a late step in protein secretion. Herein, we characterize a mammalian homolog of l(2)gl, called Mlgl, in t...

In confluent monolayers of the dog kidney epithelial cell line Madin-Darby canine kidney (MDCK) assembly of RNA enveloped viruses reflects the functional polarization of the cells. Thus, influenza, Sendai, and Simian virus 5 bud from the apical (free) surface, while vesicular stomatitis virions (VSV) are assembled at basolateral plasma membrane domains (Rodriguez-Boulan, E., and D.D. Sabatini, ...