A protocol is described for individually collecting two populations of sperm cells, Svn and Sua, from pollen of Plumbago zeylanica. Pollen grains were burst in 10 mM MOPS buffer containing 0.8 M mannitol (pH 4.6). Paired sperm cells released from pollen were separated using a microinjector. Svn and Sua were then collected individually with a microinjector, based upon known size differences. Col...

The process of document annotation for the Semantic Web is complex and time consuming, as it requires a great deal of manual annotation. Information extraction from texts (IE) is a technology used by some very recent systems for reducing the burden of annotation. The integration of IE systems in annotation tools is quite a new development and there is still the necessity of thinking the impact ...

The process of document annotation for the Semantic Web is complex and time consuming, as it requires a great deal of manual annotation. Information extraction from texts (IE) is a technology used by some of the most recent systems for actively supporting users in the process and reducing the burden of annotation. The integration of IE systems in annotation tools is quite a new development and ...