Metagenome, a mixture of different genomes (as a rule, bacterial), represents a pattern, and the analysis of its composition is, currently, one of the challenging problems of bioinformatics. In the present study, the possibility of evaluating metagenome composition by DNA-marker methods is investigated. These methods are based on using primers, short nucleic acid fragments. Each primer picks ou...

Music emotion recognition systems have been shown to perform well for musical genres such as film soundtracks and classical music. It seems difficult, however, to reach a satisfactory level of classification accuracy for popular music. Unlike genre, music emotion involves complex interactions between the listener, the music and the situation. Research on MER systems is handicapped due to the la...

Techniques such as arbitrarily primed polymerase chain reaction (APPCR) (11), random amplified polymorphic DNA (RAPD) (12) and DNA amplification fingerprinting (DAF) (7) are powerful tools for genetic mapping, taxonomic and phylogenetic investigations and for the detection of various types of DNA damage and mutation (2,3). Nevertheless, the arbitrary nature of the DNA polymerization catalyzed i...

A final common pathway has been devised for analysis of de novo mutation at any CpG site. Artificial restriction sites can be introduced in known DNA sequences by using either or both sense and antisense mismatched PCR primers. Sitting of the primers directly adjacent 5' and 3' to the CpG site yields a 52-bp PCR product, resulting from the sum of the two 25-mer oligonucleotides plus the two int...

Microsatellites or simple sequence repeats provide an attractive source of genetic polymorphisms for both mammals and plants (1). Microsatellites can be detected by PCR using minute amounts of starting material and they provide codominant markers with a high degree of allelic polymorphism. However, these markers are obtained through a difficult and labor-intensive procedure. In contrast, RAPD m...

A method has been developed to prepare random DNA fragments using PCR. First, two cycles are carried out at 16 degrees C with the Klenow's fragment and oligonucleotides (random primers) with random 3'-sequences and the 5'-constant part containing the site for cloning with the site-specific endonuclease. The random primers can link to any DNA site, and random DNA fragments are formed during DNA ...

Background: Acer griseum Pax is an endangered species endemic to China with both ornamental and economic value. However, the lack of information on its genome size characteristics hinders further work at level. Methods: This paper applied bioinformatics methods predict patterns A. genome, providing important basis for formulating whole-genome sequencing scheme. study als...

PCR-based fingerprinting using random amplified polymorphic DNA (RAPD) has been used widely for genome identification. In this study, 13 Salmonella Typhi strains were isolated from typhoid patients from Aswan, Cairo, Fayoum, and Monofya Governorates of Egypt. The isolates, along with three reference strains, i.e., O901, H901, and Ty2 were subjected to whole genome typing by RAPD PCR. Three RAPD...

A Burkholderia cepacia population naturally occurring in the rhizosphere of Zea mays was investigated in order to assess the degree of root association and microbial biodiversity at five stages of plant growth. The bacterial strains isolated on semiselective PCAT medium were mostly assigned to the species B. cepacia by an analysis of the restriction patterns produced by amplified DNA coding for...