Intradermal inoculation of metacyclic forms of Trypanosoma congolense propagated in vitro caused skin reactions in goats similar to the local skin reaction (chancre) induced by the bite of an infected tsetse fly. The onset, size and duration of these local skin reactions were dose-dependent. Whereas one cultured metacyclic T. congolense was sufficient to cause a local skin reaction in a goat, o...

Biological parameters of five Trypanosoma cruzi strains from different sources were determined in order to know the laboratory behaviour of natural populations. The parameters evaluated were growth kinetics of epimastigotes, differentiation into metacyclic forms, infectivity in mammalian cells grown in vitro and parasite susceptibility to nifurtimox, benznidazole and gentian violet. Differences...

Leishmania are protozoan parasites that cause several human diseases called leishmaniases, which can display very different clinical aspects (Peters and Killick-Kendrick, 1987b). The life cycle of Leishmania involves two kinds of hosts: dipteran insects (sandflies) and several mammals, in which they adopt a motile, flagellated, promastigote form and a non-motile, amastigote form with a very sho...

This question is rather old [3]: Which groups having periodic cohomology can act freely on some homotopy sphere? The first nontrivial restriction was supplied by Milnor in [3]. Every element of order two must lie in the center. Swan [ô] showed that any group with periodic cohomology acts freely on a c.w. complex of the homotopy type of sphere. If the group has odd order then it is metacyclic. T...

The metacyclic trypanosomes of a Trypanosoma brucei brucei clone (ILTat 2.1) were analysed with regard to their variable antigen (VAT) composition using monoclonal antibodies. The metacyclic population was antigenically heterogeneous. Despite the heterogeneity, however, the overall VAT composition of the metacyclic population appeared to be limited in number. A similar pattern of reactivity was...

Bloodstream form trypomastigotes of four cloned stocks of Trypanosoma congolense from West Africa were successfully adapted to continuous in vitro culture at 28 degrees C using bovine aorta endothelial cell monolayers and Eagle's minimum essential medium supplemented with 20% normal bovine serum or foetal calf serum. The trypanosomes maintained in vitro morphologically resembled bloodstream for...

The protozoan Trypanosoma cruzi expresses multiple isoforms of the GP63 family of metalloproteases. Polyclonal antiserum against recombinant GP63 of T. cruzi (TcGP63) was used to study TcGP63 expression and localization in this organism. Western blot analysis revealed that TcGP63 is 61 kDa in epimastigotes, amastigotes, and tissue culture-derived trypomastigotes but 55 kDa in metacyclic trypoma...

This article may be used for research, teaching and private study purposes. Any substantial or systematic reproduction, redistribution , reselling , loan or sub-licensing, systematic supply or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to da...

Parasitic manipulations of host behaviour are known from a wide range of host-parasite associations. However, the understanding of these phenomena is far from complete and detailed investigation of their proximate causes is needed. Many studies report behavioural modifications, such as altered feeding rates in tsetse fly (Glossina) infected with the mature transmissible stage (i.e. metacyclic) ...

This paper aimed to verify the influence of the inoculum source (blood or metacyclic trypomastigote) and the route of inoculation (intraperitoneal or conjunctival) on the course of T. cruzi infection in dogs, using comparatively the T. cruzi strains Berenice-62 and Berenice-78. All dogs inoculated intraperitoneally became infected independently of the T. cruzi strain and source of trypomastigot...