Biochips are physically and/or electronically controllable miniaturized labs. They are used for combinatorial chemical and biological analysis in environmental and medical studies. The precise positioning of the samples on the surface of the chip in picoliter to nanoliter volumes can be done either by means of external forces (active devices) or by specific geometric patterns (passive devices)....

The need for affordable and disposable DNA biochips that can perform in situ analysis is increasing daily. Amperometric detection (AD) is one of the most popular detection techniques used in DNA sensing. An important component of these biochips is the working electrode that needs to be carefully designed to achieve good sensitivity. At present, most AD biochips use platinum (Pt) or gold (Au), s...

Microfluidic bioartificial organs allow the reproduction of in vivo-like properties such as cell culture in a 3D dynamical micro environment. In this work, we established a method and a protocol for performing a toxicogenomic analysis of HepG2/C3A cultivated in a microfluidic biochip. Transcriptomic and proteomic analyses have shown the induction of the NRF2 pathway and the related drug metabol...

Digital microfluidic biochips are modification many areas of Biochemistry, Biomedical sciences, and Microelectronics. It is also known as ‘Lab-on-a-Chip’ for its recognition as an alternative to laboratory experiments. In recent times, because of urgency and cost efficacy, several assay operations are required to be performed at the same time. So, parallelism is a must in designing biochips. Ha...

Purpose: Early diagnosis of prostate cancer (PCa) is essential for the prevention of metastasis and for early treatment; therefore, we aimed to develop a simple, accurate, and multi-analyte assay system for early PCa diagnosis in this study. Experimental design: We fabricated three kinds of biochips then integrated into microfluidic device for simultaneous detection of vascularendothelial growt...

Biochips, or digital labs-on-chip, are developed with the purpose of being used by laboratory technicians or biologists in laboratories or clinics. In this article, we expand this vision with the goal of enabling everyone, regardless of their expertise, to use biochips for their own personal purposes. We developed OpenDrop, an integrated electromicrofluidic platform that allows users to develop...

Biochips are physically and/or electronically controllable miniaturized labs. They are used for combinatorial chemical and biological analysis in environmental and medical studies, e.g., for high throughput screening, hybridization and sequencing in genomics, protein profiling in proteomics, and cytometry in cell analysis. The precise positioning of the samples (e.g., DNA or proteins) on the su...

We have analyzed transcriptomic, proteomic and metabolomic profiles of hepatoma cells cultivated inside a microfluidic biochip with or without acetaminophen (APAP). Without APAP, the results show an adaptive cellular response to the microfluidic environment, leading to the induction of anti-oxidative stress and cytoprotective pathways. In presence of APAP, calcium homeostasis perturbation, lipi...