Altered plasma neutrophil microparticle levels have recently been implicated in a number of vascular and inflammatory diseases, yet our understanding of their actions is very limited. Herein, we investigate the proteome of neutrophil microparticles in order to shed light on their biological actions. Stimulation of human neutrophils, either in suspension or adherent to an endothelial monolayer, ...

Digital holography is an effective 3D imaging technique, with the potential to be used for particle size measurements. A digital hologram can provide reconstructions of volume samples focused at different depths, overcoming the focusing problems encountered by other imaging based techniques. Several particle analysis methods discussed in the literature consider spherical particles only. With th...

BACKGROUND Injury to the mesothelial layer of the peritoneal membrane during peritoneal dialysis (PD) is implicated in loss of ultrafiltration capacity, but there are no validated biomarkers for mesothelial cell injury. Microparticles (MPs) are 0.1 to 1.0 µm membrane vesicles shed from the cell surface following injury and are sensitive markers of tissue damage. Formation of MPs in the peritone...

objective(s) theophylline, a xanthenes derivative, is still widely used as an effective bronchodilator in the management of asthmatic patients. it is used both as a prophylactic drug and to prevent acute exacerbations of asthma. the aim of study was to formulate and evaluate effect of the microencapsulation of theophylline loaded nanoparticles on the reduction of burst release. materials and me...

We demonstrate controlled manipulation of large quantities of microparticles on a surface using inertial forces. Motion is induced by applying a periodic parabolic wave form to a shear-polarized piezoelectric plate coupled to a substrate on which the particles reside. Particles move in steps of 10 to 50 nm per cycle, and the particle motion is mass selective. Particle velocity is varied by chan...

Microparticles express phospholipids and support thrombin generation, which increases with age [1,2]. In a recently published study, we showed age-dependent changes in thrombin generation parameters in a healthy infant population aged 1-24 months [3]. The aim of this present study was to compare the levels of both indirect microparticle activity and thrombin generation parameters of healthy inf...

Articles you may be interested in Multiphysics of ionic polymer–metal composite actuator Thickness dependence of curvature, strain, and response time in ionic electroactive polymer actuators fabricated via layer-by-layer assembly We have employed the easy-to-scale-up method of spray-coating in combination with layer-by-layer self-assembly technique to fabricate ionic electroactive polymer actua...

Microparticles represent a heterogeneous population of vesicles with a diameter of 100 to 1000 nm that are released by budding of the plasma membrane and express antigens specific of their parental cells. Although microparticle formation represents a physiological phenomenon, a multitude of pathologies are associated with a considerable increase in circulating microparticles, including inflamma...

In this study we have evaluated the influence of biomaterial nano-topography on platelet adhesion and activation. Nano-porous alumina membranes with pore diameters of 20 and 200 nm were incubated with whole blood and platelet rich plasma. Platelet number, adhesion and activation were determined by using a coulter hematology analyzer, scanning electron microscopy, immunocytochemical staining in ...

Biocompatible, biodegradable polymers are commonly used as excipients to improve the drug delivery properties of aerosol formulations, in which acetalated dextran (Ac-Dex) exhibits promising potential as a polymer in various therapeutic applications. Despite this promise, there is no comprehensive study on the use of Ac-Dex as an excipient for dry powder aerosol formulations. In this study, we ...