To address the diversity of microplate washing requirements, BioTek has developed the ELx405TM, a family of microplate washers that provides versatility, as well as dedicated capabilities. The ELx405 Select Microplate Washer uses a patented manifold design which provides independent control of aspirate and dispense tube location and height enabling bubble free fluid dispense and overflow protec...

The most common method in the routine determination of uronic acids, the m-hydroxydiphenyl reaction, recently adapted to rapid microplate analysis, has as a main inconvenience, in any one of their modalities, interferences due to the frequent presence of proteins and neutral carbohydrates in the samples. Corresponding corrections in the literature are unsatisfactory when applied to complex matr...

Here, we present a Microsoft Windows application that has been written to emulate a microplate reader. After scanning a standard 96-well microplate, color gradations in the slots can be analyzed quantitatively and be used for the estimation of enzyme kinetics, binding assays or concentration determination. In a test, series accuracy of the program was determined. We show that the returned optic...

The micro-ELISA described in this report is simple and can give objective results for the serodiagnosis of human cysticercosis. The visual assessment of results was satisfactory. The ELISA results showed a remarkably good correlation with IHA. It gave good reproudcibility. Cross-reactions were eliminated by using whole worm antigen instead of the cysticercus.

The divalent cation, calcium, serves as a mediator for a large number of intracellular biological responses. The advent of specific fluorogenic compounds that specifically bind to calcium has allowed investigators to study changes in Ca2+ under a variety of conditions. The purpose of this application note is to demonstrate the utility of the FLx800 fluorescence microplate reader to detect rapid...

Here, we present data on the effects of emu oil, obtained from emu (Dromaius novaehollandiae) fat deposits, on melanogenesis in B16F1 murine melanoma cells. The cells were cultured in media containing different concentrations of emu oil, and the melanin content of these cells was measured using a microplate reader. Next, melanin content was measured for cells cultured with α-melanocyte-stimulat...