نتایج جستجو برای: microsatellite dna
تعداد نتایج: 521383 فیلتر نتایج به سال:
Microsatellites are short tandemly repeated DNA sequence motifs that are highly variable in most organisms. In contrast to mammals, long microsatellites (>15 repeats) are extremely rare in the Drosophila melanogaster genome. To investigate this paucity of long microsatellites in Drosophila, we studied 19 loci with exceptionally long microsatellite alleles. Inter- and intraspecific analysis show...
A population of offspring from a cross between ‘Golden Smoothee’ × ‘Shafi Abadi’ apple which was developed by two methods of controlled pollination (with and without covering after controlled pollination) was used to investigate the inheritance of microsatellite alleles and the necessity of covering in controlled pollination of apple. DNA was extracted from 60 seedlings (30 from each method) as...
Abstract Background: Hereditary non-polyposis colorectal cancer is the most common cause of early onset of hereditary colorectal cancer. In the majority of Hereditary non-polyposis colorectal cancer families, microsatellite instability and germline mutation in one of the DNA mismatch repair genes in clouding MSH2, MLH1, MSH6 and PMS2 are found. The Objective of this study was to determine th...
Sequence tagged microsatellite profiling (STMP) enables the rapid development of large numbers of co-dominant DNA markers, known as sequence tagged microsatellites (STMs). Each STM is amplified by PCR using a single primer specific to the conserved DNA sequence flanking the microsatellite repeat in combination with a universal primer that anchors to the 5'-ends of the microsatellites. It is als...
Understanding how microsatellites are distributed in eukaryotic genomes is important to clarify the differential abundance of these repeats under an evolutionary scenario. We have concatenated data from 3165 DNA sequences of 326 Bivalvia species to search for taxonomic patterns of microsatellite distribution in genomic regions of markedly different functionality. Some microsatellite motifs in b...
Paternity tests are carried out by the analysis of hypervariable short tandem repeat DNA loci. These microsatellite sequences mutate at a higher rate than that of bulk DNA. The occurrence of germline mutations at STR loci posses problems in interpretation of resulting genetic profiles. We recently analyzed 59-159 parent/child allele transfers at 13 microsatellite loci. We identified 12 mutation...
A population of offspring from a cross between ‘Golden Smoothee’ × ‘Shafi Abadi’ apple which was developed by two methods of controlled pollination (with and without covering after controlled pollination) was used to investigate the inheritance of microsatellite alleles and the necessity of covering in controlled pollination of apple. DNA was extracted from 60 seedlings (30 from each method) as...
A microsatellite-enriched library of plateau pika (Ochotona curzoniae) was constructed according to the strong affinity between biotin and streptavidin. Firstly, genomic DNA was fragmented by ultrasonication, which is a major improvement over traditional methods. Linker-ligated DNA fragments were hybridized with biotinylated microsatellite probes, and then were subjected to streptavidin-coated ...
We have developed a vectorette PCR approach to provide an improved method for isolation of microsatellite repeats. The modified procedure relies on PCR amplification using a vectorette-specific primer in combination with one of a panel of anchored dinucleotide repeat primers. The target DNA to be screened for microsatellite sequences can be from YAC, P1, cosmid, bacteriophage or plasmid clones....
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