Genetic aberrations in sarcomas 10 2.1. Cytogenetic alterations 10 2.2. Molecular genetic changes 13 2.2.1. Structural alterations of cellular proto-oncogenes 13 2.2.2. Gene amplifications 13 2.2.3. Alterations of tumor suppressor genes and loss of 17 DNA sequences 2.3. Hereditary predisposition 18 3. DNA amplification 19 3.1. Structure and mechanisms of DNA amplification 20 4. Methods for dete...

BACKGROUND Echinococcus granulosus, Taenia multiceps, Taenia ovis and Taenia hydatigena are among the most prevalent taeniid species of dogs. These tapeworms infect ruminant and humans as intermediate hosts and domestic/wild carnivores as the definitive hosts. Molecular tools using hypervariable microsatellite regions might provide more information about parasite variation. Highly variable and ...

PURPOSE Microsatellite instability (MSI) testing of colorectal cancer tumors is used as a screening tool to identify patients most likely to be mismatch repair (MMR) gene mutation carriers. We wanted to examine which microsatellite markers currently used to detect MSI best predict early-onset colorectal cancer caused by germ-line mutations in MMR genes. EXPERIMENTAL DESIGN Invasive primary tu...

one of the traits of the greatest financial impact on sheep production is the number of lambs weaned per ewe with fecb considered as the most well known major gene concerned with this trait. in order to conduct a marker assisted introgression (mai) program the presence of fecb responsible mutation in afshari sheep breeding flock was surveyed. direct test to detect fecbb allele was conducted on ...

The identification of informative in silico polymorphic genomic and genic microsatellite markers by comparing the genome and transcriptome sequences of crop genotypes is a rapid, cost-effective and non-laborious approach for large-scale marker validation and genotyping applications, including construction of high-density genetic maps. We designed 1494 markers, including 1016 genomic and 478 tra...

Linkage disequilibrium (LD) is bases of genomic selection, genomic marker imputation, marker assisted selection (MAS), quantitative trait loci (QTL) mapping, parentage testing and whole genome association studies. The Particular alleles at closed loci have a tendency to be co-inherited. In linked loci this pattern leads to association between alleles in population which is known as LD. Two metr...

We present the first molecular marker linkage map for Musca domestica containing 35 microsatellite plus six visible markers. We report the development of 33 new microsatellite markers of which 19 are included in the linkage map. Two hundred and thirty-six F2 individuals were genotyped from three crosses yielding a linkage map consisting of five linkage groups that represent the five autosomes o...

Cystic fi brosis is an autosomal recessive disease. Its incidence in Cuba is 1 in 5000 live births. The molecular cause underlying this disease is related to mutations in the regulatory gene encoding the cystic fi brosis transmembrane regulator (CFTR). In this study, the techniques for the study of IVS17bTA microsatellite marker were standardized, and the most frequent mutations in the CFTR gen...

During microsatellite marker development, researchers must choose from a pool of possible primer pairs to further test in their species of interest. In many cases, the goal is maximizing detectable levels of genetic variation. To guide researchers and determine which markers are associated with higher levels of genetic variation, we conducted a literature review based on 6782 genomic microsatel...

Although much research has been conducted to characterize microsatellites and develop markers, the distribution of microsatellites remains ambiguous and the use of microsatellite markers in genomic studies and marker-assisted selection is limited. To identify microsatellites for cotton research, we mined 100,290, 83,160, and 56,937 microsatellites with frequencies of 41.2, 49.1, and 74.8 micros...