The development of the pollen grain wall in Xiphidium coeruleum (Haemodoraceae) was studied using TEM and cytochemical staining techniques. Microsporocyte ontogeny initiates with the degradation of the cellulosic cell wall and subsequent deposition of a thick callosic cell wall. Following callose deposition, successive meiosis occurs, resulting in a tetragonal tetrad of microspores. during meio...

Under specific stress treatments (cold, starvation), in vitro microspores can be induced to deviate from their gametophytic development and switch to embryogenesis, forming haploid embryos and homozygous breeding lines in a short period of time. The inductive stress produces reactive oxygen species (ROS) and nitric oxide (NO), signalling molecules mediating cellular responses, and cell death, m...

Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four color-related genes were identified using high resolution melting (HRM) curves of their PCR products....

To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would signif...

COMPARISON of microsporogenesis and microspore development A in a triploid Tradescantia has shown significant deviations from the normal diploid behavior. Occasional dicentric chromatids a t meiosis are found in diploid Tradescantias, but inversion crossovers in the triploid are much more frequent. An analysis of microspore development in a triploid Tradescantia shows that chromosome number has...

Aspterric Acid, 6-Hydroxymellein, Arabidopsis thaliana, Aspergillus terreus Aspterric acid (1) and 6-hydroxymellein (2), inhibitors of pollen development in Arabidopsis thaliana, have been isolated from the fungus Aspergillus terreus. 1 and 2 inhibited the pollen development at concentrations of 38 and 52 microM, respectively. The microscopic examination of pollen development suggested that the...

Here is reported a novel histological marker line that strongly and specifically labels the male gametophyte in Arabidopsis flowers, from the uninucleate microspore to the pollination stage. In this marker line, the expression of the UidA and GFP marker genes is driven by the promoter of the At5g17340 Arabidopsis gene, a gene highly expressed in the male gametophyte. Strong GUS histochemical st...

In plant breeding studies, dihaploidization method is used to obtain homozygous pure lines develop varieties in a shorter time. Dihaploidization applied most types of vegetables such as wheat and corn, well vegetable species. Androgenesis methods (anther microspore culture) gynogenesis parthenogenesis (ovula ovary are haploid plants. One the androgenetic methods, shed-microspore culture has bee...

Sporopollenin is the main constituent of the exine layer of spore and pollen walls. The anther-specific chalcone synthase-like (ASCL) enzyme of Physcomitrella patens, PpASCL, has previously been implicated in the biosynthesis of sporopollenin, the main constituent of exine and perine, the two outermost layers of the moss spore cell wall. We made targeted knockouts of the corresponding gene, PpA...