One of the most sensitive and specific methods for quantitative estimation of deoxyribonucleic acid (DNA) is the microbiological one developed by Hoff-J0rgensen (1951, 1954). This method is l)ased on the fact that Thermobacterium acidophilum (Lactobacillus acidophilus) strain R 26 Orla Jensen is unable to grow in the absence of deoxyribosides. The DNA containing sample is digested with deoxyrib...

BACKGROUND Gastric juice vitamin C may be protective against gastric carcinogenesis but concentrations are significantly reduced by Helicobacter pylori infection. We investigated the in vitro effects of vitamin C at concentrations comparable with those found in gastric juice on gastric cancer cells and H pylori. METHODS Gastric cancer cell lines and various H pylori strains were treated with ...

Abstract Background and Purpose: In traditional medicine, herbal products still remain the principal source of pharmaceutical agents. The present study aimed to investigate the antifungal effects of Echinophora platyloba and Rosmarinus officinalis extracts on C. albicans species. Materials and Methods: The aqueous and ethanolic leaf extracts of E. platyloba and R. officinalis, collected f...

abstract background and purpose: in traditional medicine, herbal products still remain the principal source of pharmaceutical agents. the present study aimed to investigate the antifungal effects of echinophora platyloba and rosmarinus officinalis extracts on c. albicans species. materials and methods: the aqueous and ethanolic leaf extracts of e. platyloba and r. officinalis, collected from th...

The function of SIRT1 in the proliferation and osteoblastic differentiation of dental stem cells is unclear. The aim of this study was to assess the roles of SIRT1 in these processes using periodontal ligament stem cells (PDLSCs) and stem cells from apical papilla (SCAPs). A defined concentration of resveratrol, an SIRT1 activator, or nicotinamide, an SIRT1 inhibitor, was administered to PDLSCs...

BACKGROUND AND PURPOSE In traditional medicine, herbal products still remain the principal source of pharmaceutical agents. The present study aimed to investigate the antifungal effects of Echinophora platyloba and Rosmarinus officinalis extracts on C. albicans species. MATERIALS AND METHODS :The aqueous and ethanolic leaf extracts of E. platyloba and R. officinalis, collected from the mounta...

A sensitive method based on PCR followed by liquid-phase hybridization for detection of enterovirus and rhinovirus RNAs in clinical specimens and cell culture supernatants is described. RNA was extracted from stool samples, throat swabs, nasopharyngeal aspirates, cerebrospinal fluid, urine, and plasma with a commercial phenol-guanidinium-chloroform reagent and purified on a polysulfone membrane...

The buffering capacity of tears collected from six young, healthy subjects was assessed using a microtitration technique. Each subject provided, on six separate occasions, about 100 microliter of tears, collected in small amounts and with minimal mechanical stimulation over several hours. The pH of the total stirred pool of tears from each subject was determined at the outset. This pool of tear...

The pH of airway epithelial secretions influences bacterial killing and mucus properties and is reduced by acidic pollutants, gastric reflux, and respiratory diseases such as cystic fibrosis (CF). The effect of acute acid loads depends on buffer capacity, however the buffering of airway secretions has not been well characterized. In this work we develop a method for titrating micro-scale (30 μl...

One of the most sensitive and specific methods for quantitative estimation of deoxyribonucleic acid (DNA) is the microbiological one developed by Hoff-J0rgensen (1951, 1954). This method is l)ased on the fact that Thermobacterium acidophilum (Lactobacillus acidophilus) strain R 26 Orla Jensen is unable to grow in the absence of deoxyribosides. The DNA containing sample is digested with deoxyrib...