We describe the development and validation of a qPCR assay to detect Poecilia reticulata, highly invasive species freshwater fish Caribbean islands, through environmental DNA (eDNA) sampling. Originating from Trinidad, this is detrimental countless native tropical communities. A assay, consisting set primers fluorescent probe, amplifying 214 base pair target region mitochondrial Cytochrome B ge...

background: angiostrongylus cantonensis is a zoonotic public health concern that causes human severe eosinophilic meningitis in southeast asia and china. as a medically important intermediate host of a. cantonensis , bellamya lithophaga (gastrop-oda: viviparidae) is often confused with other morphologically similar sibling spe-cies of genus bellamya , such as b. aeruginosa and b. purificata in ...

One of the top species in aquaculture sector, known as striped catfish or Pangasianodon hypophthalmus, is an important and valuable freshwater fish many countries. Due to high demand for this species, their number has declined "threatened" levels. Hence, purpose study analyse genetic variation wild cultured collected from five producers Asian countries; Thailand, Vietnam, Indonesia, India, Phil...

Hemiculter leucisclus , as a widely distributed freshwater fish in China, provides an interesting model to explore the impact of drainage evolution and geologic history Pleistocene on diversification patterns. We collected mitochondrial cytochrome b (cytb) gene recombination activating 2 (RAG2) from 1,070 individuals 59 sampling locations. Phylogenetic population genetic approaches were used de...

We tested the mitochondrial DNA cytochrome b gene-based (1065 bp.) phylogeny and genetic diversity of tooth-carp fish (Aphaniidae Hoedeman, 1949 family), many which are endemic, with a very high species in Anatolia. A total 107 individuals were studied from 29 stations belonging to 19 Aphaniid species. For Cytb gene, 44 haplotypes identified, all species-specific. The phylogenetic relationships...

Three new species, Cobitis almadae sp. nov., atlantica and mellaria nov. are described on the basis of morphological genetic traits. is restricted to Sizandro Drainage in Portugal can be distinguished from other species through a combination morphometric traits including large low peduncle depth, lateral ethmoid (suborbital spine) well developed with long narrow mediocaudal, laterocaudal medior...

The recent demonstration that certain mitochondrial subunits of cytochrome oxidase (CO) are regulated by neuronal activity has stimulated interest in the molecular processes that coordinate nuclear and mitochondrial gene expression following synaptic stimulation. We have studied the constitutive expression and activity-guided regulation of cytochrome b (cyt b), a gene that is encoded by mitocho...

A study of the mealybug genus Planococcus Ferris, 1950 (Hemiptera, Coccomorpha, Pseudococcidae) known from China is presented and 12 species are recognised. Of these, camelliae Zhang, sp. nov. described as new to science based on morphology adult female, P. bambusifolii (Takahashi, 1951) recorded for first time. Molecular analyses mitochondrial gene cytochrome c oxidase subunit I (COI) a key in...

Since October 2019, chinch bugs have been detected in large sunken dead patches of St. Augustinegrass (Stenotaphrum secundatum) lawns different locations Lisbon and Setubal districts, Portugal. Based on morphological molecular studies, using mitochondrial Cytochrome C Oxidase subunit I (COI) gene, the collected insects were identified as Blissus insularis (Hemiptera, Heteroptera, Blissidae). Th...

To reconstruct the phylogenetic position of the extinct cave lion (Panthera leo spelaea), we sequenced 1 kb of the mitochondrial cytochrome b gene from two Pleistocene cave lion DNA samples (47 and 32 ky B.P.). Phylogenetic analysis shows that the ancient sequences form a clade that is most closely related to the extant lions from Africa and Asia; at the same time, cave lions appear to be highl...