Methods are presented for obtaining simultaneously or separately two populations of cells from human peripheral blood, lymphocytes and monocytes, both of which are required to obtain blastogenesis and DNA synthesis in human leukocyte cultures. A simple 5-mm centrifugation of heparinized blood yields a mononuclear leukocyte culture fluid containing 70-90#{176}/olymphocytes with few granulocytes ...

Th17 response following in vitro exposure to mercury with and without n-3 long chain polyunsaturated fatty acids peripheral blood mononuclear cells from systemic lupus erythematosus patients compared healthy controls - Volume 82 Issue OCE4

We examined the relationships among CD4+-T-cell counts, spontaneous apoptosis, and Fas expression among peripheral blood mononuclear cells obtained from human immunodeficiency virus type 1 (HIV-1)-infected patients. After 2 days of incubation, propidium iodide DNA staining and flow cytometry revealed that peripheral blood mononuclear cells from subjects with the lowest CD4+-cell numbers (0 to 9...

Objective: The aim of this study is to observe the homing and distribution of the foreign multidrug resistance gene 1 (mdr1) in gene therapy. Method: After the bone marrow mononuclear cells of male BABL/C which was transferred with retrovirus-mediated mdr1 were transplanted into female Babl/c which was conducted radiotherapy with 60Co-γ, the expression and distribution of foreign mdr1 in the bo...

Platelets induce generation of procoagulant tissue factor activity (TFa) by mononuclear leukocytes, and also enhance the TFa induced by endotoxin. Our present investigation demonstrated that arachidonic acid, which by itself had no effect on mononuclear TFa, greatly enhanced platelet-induced TFa. The effect was concentration dependent for both platelets and arachidonate (1-20 microM); other fat...

OBJECTIVES Stem cell transplant can induce vasculogenesis and improve the blood supply to an ischemic region, offering hope for chronic lower extremity ischemic diseases. Bone marrow mononuclear cells are one of the sources for stem cell transplants. We sought to observe the safety and efficacy of autologous bone marrow mononuclear cells transplant for treating critical limb ischemia. MATERIA...

Soluble plasma tissue factor (TF) circulates in picomolar concentrations in healthy individuals and increases in a wide spectrum of diseases. This study tests the hypothesis that both truncated TF (rsTF) or soluble plasmaTF (pTF) in low concentration combine with monocytes or platelets to convert factorVII (fVII) to fVIIa. Both rsTF (33 kDa) and pTF (47 kDa), obtained from pericardial wounds of...

The effects of mononuclear cells from nontransfused and transfused dogs on the growth of erythroid colonies (EC) from DLA-identical littermates were studied. To this purpose, marrow samples were cultured both before and after one to three transfusions from the DLA-identical littermate. Mononuclear cells obtained before transfusions and cocultured with autologous marrow or marrow from DLAidentic...

AIM To investigate the hypothesis that complement mediates the recruitment of mononuclear osteoclast precursors to the exposed mineralised bone surface. METHODS Synthetic hydroxyapatite was incubated in vitro with fresh human serum, with and without complement activation inhibitors. Assays for complement components and the generation of the C3 breakdown product C3d were done. C3 deposition in...

Coxsackievirus group B type 3 (CVB3) is an important cause of viral myocarditis. The infiltration of mononuclear cells into the myocardial tissue is one of the key events in viral myocarditis. Immediately after CVB3 infects the heart, the expression of chemokine(s) by infected myocardial cells may be the first trigger for inflammatory infiltration and immune response. However, it is unknown whe...