We have examined the capacity of monocytes from patients with leprosy to undergo activation and the capacity of mononuclear cells from these patients to incorporate [3H]thymidine and produce monocyte-activating cytokines. Monocytes from patients with either lepromatous or tuberculoid leprosy were activated by concanavalin A (Con A)-induced mononuclear cell supernatants generated from the leukoc...

introduction: an investigation of bone, cartilage and fat differentiation potentials of canine bone marrow-derived mesenchymal stem cells. materials and methods: bone marrow aspirates were obtained from 10 canine’s iliac crest under general anesthesia. each sample was separately loaded on lymphodex and centrifuged at 1200 rpm for ten minutes to obtain mononuclear cell fraction. 5×104 cell/cm2 o...

Hepatocellular carcinoma (HCC) is frequently associated with infiltrating mononuclear inflammatory cells. We performed laser capture microdissection of HCC-infiltrating and noncancerous liver-infiltrating mononuclear inflammatory cells in patients with chronic hepatitis C (CH-C) and examined gene expression profiles. HCC-infiltrating mononuclear inflammatory cells had an expression profile dist...

P. vermicola is an opportunistic pathogen of human and animals. To estimate the pathogeneses P.vermicola in mice infected orally I/P with 1x108 CFU/ml).The histopathological changes at 24 hrs showed infiltration inflammatory cells apoptosis hepatocytes liver, around a congested blood vessel interstitial tissue kidney; lung revealed congestion vessels, alveolar emphysema; intestine appeared mono...

The fine structural characteristics and phagocytic properties of peroxidase-positive and peroxidase-negative cells in rat hepatic sinusoids were investigated. Cells with a positive peroxidase reaction in the endoplasmic reticulum and the nuclear envelope make up approximately 40% of cells in rat hepatic sinusoids and have abundant cytoplasm containing numerous granules and vacuoles, and occasio...

We examined the relationships among CD4+-T-cell counts, spontaneous apoptosis, and Fas expression among peripheral blood mononuclear cells obtained from human immunodeficiency virus type 1 (HIV-1)-infected patients. After 2 days of incubation, propidium iodide DNA staining and flow cytometry revealed that peripheral blood mononuclear cells from subjects with the lowest CD4+-cell numbers (0 to 9...

The multinucleated bone-resorbing osteoclast has a hematopoietic origin. We have demonstrated previously that osteoclasts are derived from the monocytic lineage by fusion of mononuclear macrophage precursors. Using an in vitro-osteoclast differentiation model derived from pure populations of chick macrophage cultures, osteoclast-like multinucleated giant cells (MNGCs) can be formed by fusion fo...

Mononuclear phagocytes consisting of monocytes, macrophages, and DCs play a complex role in tumor development by either promoting or restricting growth. Cutaneous squamous cell carcinoma (cSCC) is the second most common nonmelanoma skin cancer arising from transformed epidermal keratinocytes. While present at high numbers, tumor-infiltrating resident myeloid cells formation cSCC largely unknown...

Objective: To explore the expression and clinical significance of miR-155 miR-146a in psoriasis patients from different sources. Methods: The levels vulgaris tissues, peripheral blood mononuclear cells, serum were detected by QRT-PCR method 30 with healthy subjects as normal control group, correlation between samples sources was analyzed. And after treatment. Results: skin tissue, cells signifi...

When mononuclear cells infiltrating rabbit testes infected with Treponema pallidum were cocultured with autologous or homologous peripheral blood lymphocytes spontaneous stimulation and that induced by concanavalin A were suppressed. The inhibition was not due to the cytotoxic effect of the mononuclear cells or to their interference with the active site of concanavalin A (competitive inhibition...