Nodal segments from six years old plants of Bauhinia variegata L. were cultured on Murashige and Skoogs (MS) medium supplemented with different concentration and combinations of BAP and IBA. Explants culture in MS basal medium supplemented with 5mg/1 BAP showed highest rate of shoot multiplication. When in vitro shoots were inoculated on the MS basal medium supplemented with IBA 2mg/l, profusse...

Background: Stevia rebaudiana Bertoni, an important anti-diabetic medicinal plant, becomes an inevitable alternative to sugar. Due to the propagation difficulties, tissue culture is the best alternative for rapid mass propagation of stevia plants. Objective: The present study was conducted to optimize a protocol for rapid micropropagation of Stevia rebaudiana by shoot tip explants and to inv...

This study was conducted to evaluate the effect of plant growth regulators on in vitro shoot multiplication, vitrification and rooting of two carnation cultivars (Eskimo Mogr and Innove Orange Bogr). Isolated axillary buds were cultured on MS medium supplemented with different levels of Benzyl amino purine (BAP) or kinetin (Kin) in combination with 0.2 mg/l NAA and shoot multiplication and vitr...

Shoot tips and nodal segment explants to Rauwolfia serpentina when cultured on MS medium containing varying concentration of Benzyl adenine purine (BAP) and Benzyl adenine purine in combination with Indole butyric acid (IBA) produced multiple shoots. Maximum multiple shoots (85.6%) were found in static MS medium supplemented with 5.0 mg/ 1 BAP and 0.5 mg / 1 IBA along with 2.5% sucrose and 0.85...

The rapid direct and repetitive somatic embryogenesis in Coffea arabica and C. canephora genotypes was tested on Murashige and Skoog medium (MS) containing thidiazuron (TDZ) (1-phenyl-3-(1,2,3,-thiadiazol-5-yl)urea) in concentrations of 2.27–11.35s M. Segments taken from cotyledon leaf, first leaf and stalk of regenerated plantlets produced clusters of somatic embryos directly from cut portions...

1. Asparagus racemosus is an herb used as a rasayana in Ayurveda. The root of A racemosus is popular for its various medicinal uses such as appetizer, stomachic, tonic aphrodisiac galactogogus etc. In this experiment we have found the shoot induction after 3 weeks when Nodal explants segments were cultured in basal medium supplemented with BAP, Kin and Ads and Shoots induction on MS basal mediu...

Factors like slow and seasonal multiplication and infection with pathogens have constrained the use of conventional plant propagation methods, thus lead to development of new and novel methods of propagation like in vitro multiplication which ensures the production of virus and disease free elite planting material in large numbers. In the present work, in vitro regeneration protocol has been st...

Strawberry is a valuable, nutritious, and economically important fruit all over the world including Bangladesh. Therefore, there is a demand to develop a suitable variety of strawberry. For this purpose, leaf explants from in vitro grown strawberry plantlets were cultured onto MS medium supplemented with different concentrations and combinations of 2,4-D, NAA and BA for callus induction. The mo...

koelreuteria elegans, popularly known as “flame gold” is an ornamental tree. in vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified ms medium. the highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-d from leaf segments. calli transferred in 1.5 mg/l bap resulted in efficient shoot regeneration...