Six strains of chloramphenicol (CM)-resistant endospore-forming bacteria, which can grow in the presence of 100 microgram/ml of CM, were isolated and identified as Bacillus badius. Mechanism of CM-resistance in one of the isolated strains, Bacillus badius 211, was investigated. No inactivation of CM was demonstrated when the strain was grown in nutrient broth containing 100 microgram/ml of CM, ...

gum of butea monosperma is used to treat microbial and fungal infections in folk medicine. to validate this use, the in- vitro antimicrobial activity of petroleum ether and alcoholic extract of butea monosperma gum was evaluated against various microbial strains such as staphylococcus aureus, bacillus subtilis, bacillus cereus, salmonella typhimurium , pseudomonas aeuriogenosa, escherichia coli...

the root and flowers of butea monosperma (lam.) were extracted with methanol. extensive chromatographic separation and purification with the organic solvents was done. four phytochemicals were separated and their structures were established based on various spectroscopic techniques. isolated crude extract was subjected for antibacterial activity against gram-negative and gram-positive bacteria ...

13 strains of bacteria were isolated from 12 shoes that were worn by children aged 6 to 12 for more than half a year. Through morphological observation, physiological and biochemical measurements, as well as 16SrRNA sequence analysis, the bacteria were identified as follows: Bacillus licheniformis, Bacillus subtilis (5 subspecies), Bacillus spore (3 subspecies), Bacillus anthracis, Staphylococc...

Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.

A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by c...

Studies were carried out on the decolorization of azo dyes by Bacillus species from effluent of textile industries. About 10 bacterial strains were isolated from the effluent of textile industries, Bacillus sp. showed remarkable ability in decolorizing the widely utilized azo dyes. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicate that these s...

The world potato is facing major economic losses due to disease pressure and environmental concerns regarding pesticides use. This work aims at addressing these two issues by isolating indigenous bacteria that can be integrated into pest management strategies. More than 2,800 strains of Bacillus-like and Pseudomonas-like were isolated from several soils and substrates associated with potato agr...

background one group of significant enzymes produced by bacillus genus are alkaline proteases with several important applications in the daily life as well as common industries such as food, detergents, leather, alcohol and beer production and medical and sanitary industries, beside wastewater treatment, , biotransformation, hydrolyzed proteins and oil manufacturing. objectives in this paper, k...