نتایج جستجو برای: multiplex pcr mpcr

تعداد نتایج: 186974  

Journal: :Jundishapur Journal of Microbiology 2022

Background: Clostridium spp. spores are resistant to many factors, including alcohol-based disinfectants. The presence of clostridial in a hospital environment may lead infection outbreaks among patients and health care workers. Objective: This study is aimed detect the aurology using C diff Banana Broth™ assess antibiotic sensitivity toxinotypes isolates. Methods: After diagnosing COVID-19 med...

2016
A Santos A Almeida J M Correia Da Costa M Lourenco

Contaminated food and water are usual vehicles for bacterial pathogens transmission. According to EFSA they promote foodborne illness. Salmonella sp., Campylobacter sp., Listeria monocytogenes, Escherichia coli and Staphylococcus aureus are the most foodborne pathogens reported. Bacterial contamination levels remain at high level, particularly in Europe, despite regulatory efforts to address th...

Journal: :The new microbiologica 2009
Raffaele Del Prete Anna Maria Di Taranto Maria Rosaria Lipsi Maria Iole Natalicchio Raffaele Antonetti Giuseppe Miragliotta

The lack of rapidity and the low sensitivity and specificity of traditional laboratory methods limits their usefulness in the laboratory diagnosis of viral central nervous system (CNS) infections. This study describes the use of a commercially available multiplex polymerase chain reaction (mPCR)-based reverse hybridization assay (RHA) for the simultaneous detection of the genomes of 8 viruses a...

Journal: :acta medica iranica 0
s. sadeghian t. r. neyestani j. p. burnie

coagulase-positive and coagulase negative, methicillin-resistant staphylococci are major causes of serious nosocomial infections and it is very important to have a reliable test to detect these bacteria. a multiplex polymerase chain reaction (mpcr) was used on 100 clinical samples for simultaneous amplification of the universal bacterial, mec-a encoding the penicillin binding protein 2a, which ...

Journal: :Veterinary research 2005
Corinne Marois Jean-Paul Picault Marylène Kobisch Isabelle Kempf

The aim of the study was to analyse experimental transmission of Mycoplasma synoviae, an avian pathogen. Three experiments using specific pathogen-free day-old chicks placed in isolators were conducted. In the first experiment, the birds were introduced in an isolator previously contaminated with a M. synoviae broth culture. After 34 days, these birds were eliminated and, for the second trial, ...

Journal: :BMC Infectious Diseases 2007
Hem C Jha Harsh Vardhan Rishein Gupta Rakesh Varma Jagdish Prasad Aruna Mittal

BACKGROUND There is growing evidence that Chlamydia pneumoniae may be involved in the pathogenesis of atherosclerosis, as several studies have demonstrated the presence of the organism in atherosclerotic lesions. C. pneumoniae infections, which are especially persistent infections, have been difficult to diagnose either by serological methods or isolation of the organism from the tissue. Nuclei...

ژورنال: :پژوهش های آسیب شناسی زیستی 0
mojtaba saadati associated professor, department of biology, imam houssein university, tehran, iran shahram nazarian m.sc., department of biology, imam houssein university, tehran, iran babak barati m.sc., department of biology, imam houssein university, tehran, iran hossein mehdizadeh m.sc., department of biology, imam houssein university, tehran, iran hadi shirzad shirzad ph.d. student, department of biology, imam houssein university, tehran, iran majid sadeghizadeh associated professor, department of genetics, faculty of basic sciences, tarbiat modares university, tehran, iran abbas mosapour

هدف: مننژیت باکتریایی یکی از عفونت های جدی و گاهی کشنده است که روی سیستم اعصاب مرکزی تأثیر می گذارد. دانستن علت مننژیت ایجاد شده توسط عوامل ویروسی یا باکتریایی، به دلیل تفاوت در شدت بیماری و نیز درمان آن از اهمیت خاصی برخوردار است زیرا آنتی بیوتیک ها می توانند مانع از گسترش بعضی از این عوامل در بین مردم شوند. نیسریا مننژیتیدس و هموفیلوس آنفولانزا دو عامل مهم بیماری زا هستند که در ایجاد مننژیت ح...

Journal: :jundishapur journal of microbiology 0
hamzeh salmani department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, ir iran asaad azarnezhad department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, ir iran mohammad reza fayazi department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, ir iran arshad hosseini department of medical biotechnology, school of allied medicine, iran university of medical sciences, tehran, ir iran; department of medical biotechnology, school of allied medicine, iran university of medical sciences, tehran, ir iran. tel: +98-02186704604, fax: +98-02186552633

conclusions etec and eaggec were the most detected e. coli among stool and uti samples, emphasizing the need to dedicate more health care attention to this group. in addition, our phylogenetic study may be helpful in figuring out the infection origin and for epidemiological studies. nonetheless, more research studies with larger sample sizes are suggested for confirming our results. results ove...

چکیده    مقاومت ضدمیکروبی ایجاد شده به­واسطه تولید آنزیم­های بتالاکتاماز وسیع­الطیف (ESBLs) در اشریشیا کلی یک تهدید اساسی در کلی­باسیلوزیس ماکیان محسوب می­شود. مطالعه حاضر به شناسایی ژن­های blaTEM، blaSHVو blaOXAدر جدایه­های اشریشیا کلی به­دست آمده از کلی­باسیلوزیس طیور با استفاده از روش MPCR می­پردازد. در مجموع، 60 جدایه اشریشیا کلیاز طیور استان کرمان به­دست آمد. DNA سلولی با استفاده از کیت D...

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