Figure 1: Protein 1CRN, Crambin, is a 46 amino acid protein composed of two alpha helices and a short anti-parallel beta sheet (1(a)). When rigidity analysis is performed on the non-mutated form of Crambin, the majority of the atoms in the proteins core are part of a single large rigid cluster (shown in purple))(1(b)). KINARI-Mutagen was used to generate several in-silico mutants of crambin. Wh...

In assessing the potential health hazard a given mutagen might present to man, a number of extrapolations must be made from the test systems which are currently available. For the L5178Y mouse lymphoma cell assay system that we use, these extrapolations include: somatic versus germinal cells; single cells versus whole animals; transformed versus "normal" cells; and mouse versus human cells. All...

Mutation breeding is one of the breeding methods used successfully in durum wheat for selecting lines with increased agronomic values. This study was carried out to determine gamma rays effect and interaction between gamma-rays and the genotype of treated variety of winter wheat in mutations induction. We used physical mutagens: gamma-rays in doses 100, 150, 200, 250 Gy. Seeds of eight winter w...

In the aetiology of head and neck squamous cell carcinoma (HNSCC), smoking and heavy alcohol consumption are the main environmental risk factors. The bleomycin (BLM) sensitivity assay is believed to measure environment-related cancer risks, mainly of HNSCC. Previously, we have shown that this method is only moderately sensitive to identify individuals at high risk for developing HNSCC, due to b...

Mutations in long lived stem cells are critical events in carcinogenesis. The Dlb-1 assay detects intestinal stem cell mutation at the Dlb-I locus in Dlb-1"'' heterozygous mice by visualizing mutated clones of epithelial cells in \iiii which do not bind the lectin Dolichos bifloras agglutinin. We have used this assay to show that the food-derived heterocyclic amine 2-aminol-methyl-6-phenylimida...

This study was conducted to develop a novel herbicide resistance soybean using ethyl methanesulfonate (EMS) mutagen. In this study, 0.1% of EMS mutagen applied the [Glycine max (L.) cv Ar?soy] seeds. A single resistant mutant selected in M2 population evaluated under field and greenhouse conditions. The AHAS gene regions herbicide-resistant progeny were mapped, nucleotide changes defined confer...