The Ames assay has received widespread attention from statisticians because of its popularity and importance to risk assessment. However, investigators have yet to routinely apply modern regression methods that have been available for more than a decade. We study yet another approach, the application of nonparametric regression techniques, not as the ultimate solution but rather as a framework ...

Continuous culture systems allow for the controlled growth of microorganisms over a long period time. Here, we develop novel test mutagenicity that involves growing yeast in continuous exposed to low levels mutagen approximately 20 days. In contrast, most microorganism-based tests expose potential biological reporter at high concentration short Our improves upon sensitivity well-established Ame...

This paper surveys recent, as yet unpublished, statistical studies arising from research in genetic toxicology within the U.S. National Toxicology Program (NTP). These studies all involve analyses of data from Ames Salmonella/microsome mutagenicity tests, but the statistical methodologies are broadly applicable. Three issues are addressed: First, what is a tenable sampling model for Ames test d...

In the continuation of our study substituted isocoumarins a series novel 3-azolyl isocoumarin and their thio derivatives, including some related lactone compounds was prepared biologically profiled against C. albicans showing moderate activity with MIC values in range 4?60 ?g mL-1, general. The additional characterisation selected carried out by exploring on CYP3A4 CYP2D6 enzymes, while experim...

Mebudipine is a new dihydropyridine calcium channel blocker, synthesized in our laboratory, for treatment of hypertension. It has shown a better efficacy than other drugs in this group. For assessing the risks of this drug, certain safety tests in the preclinical stage have been performed. In this study mutagenic effect of mebudipine was evaluated using Ames assay that could assess the mutageni...