Thalictrum rhyncocarpum, a creeping plant with fern-like leaves, is used in herbal medicine to treat various infections in parts of Africa. Previously, we reported In-vitro anti-bacterial activity of different parts (root, stem bark and leaves) of the plant. In this study, bioassay-guided chromatography of an active fraction of ethanol extract of the plant against Staphylococcus aures-SG 511, P...

Cells of Mycobacterium vaccae were grown in toluene, ethanol, methyl tert-butyl ether (MTBE) and glycerol, exhibiting different degrees of tolerance towards these compounds, and being able to use both ethanol and glycerol as sole carbon and energy sources. Cellular adaptation to ethanol and MTBE led to significant changes in the membrane fatty acid (FA) composition, by modifications in the prop...

Mycobacterium vaccae 10 growing in methanol medium synthesizes two inducible alternative NAD(+)-dependent formate dehydrogenases (FDH). In the presence of molybdenum, the dominating form of the enzyme is FDHI with Mr 440 kDa and Km 0.32 mM for sodium formate. FDHI reduced ferricyanide as well as NAD+, and it was reversibly inactivated by formate. NAD+ stabilized FDHI against this inactivation. ...