The title compound, C(12)H(28)N(6), is located about an inversion center situated at the center of the -CH(2)-CH(2)- bond. The C-N bond lengths are 1.285 (2), 1.384 (2) and 1.395 (1) Å, indicating double- and single-bond character. The N-C-N angles are 114.1 (1), 119.3 (1) and 126.5 (1)°, showing a deviation of both CN(3) planes from an ideal trigonal-planar geometry.

Previous attempts to evaluate the transport of proteins across the intestinal mucosa using the caco-2 cell line have neglected the mucus layer as a barrier to drug absorption. To achieve a more accurate assessment of the absorption a coculture can be used that combines the enterocyte-like transport properties of the caco-2 cell line with the gastric mucin secretion of the HT29-MTX cell line. Un...