To investigate a1B-adrenoceptor function, we developed a phosphorothioate antisense oligodeoxynucleotide (AO) to inhibit the expression of the a1B-adrenoceptor subtype in DDT1 MF2 cells. We measured the cellular uptake of the AO and its effect on a1B-adrenoceptor mRNA expression, protein density, and coupling to phospholipase C. Cells treated with either a control oligodeoxynucleotide (CO) or m...

Since the first isolation of insulin-like proteins (ILPs) from the silkmoth, Bombyx mori in 1987, discoveries of ILPs in other insects mushroomed and propelled intense studies of the functional roles of ILPs in numerous insect species. The primary sites of ILP production are brain neuroendocrine cells, but ILPs are also produced in other tissues. Synthesis of ILPs is regulated by many factors i...

The effects of phenylephrine and methoxamine were studied on pacemaker activity in the sinoatrial node and on atrial and ventricular contractility in isolated, blood-perfused atrial and ventricular preparations of dogs. Each drug was administered directly into the cannulated sinus node artery of an isolated atrium or the anterior descending branch of the isolated left ventricle over a period of...