Somatic embryos and rooted plantlets have been regenerated from light-initiated embryogenic callus derived from mature embryos of Picea abies stored seeds. Under a 16 hour photoperiod, mature zygotic embryos were cultured on a modified half-strength Murashige-Skoog medium without NH4NO3 and supplemented with 5 mM glutamine, 4.5 pM N6-benzyladenine (BA) and 10.7 PM naphthalene-acetic acid (NAA) ...

Callus cultures derived from pith tissue of Nicotiana tabacum were grown on two media either under continuous illumination or in complete darkness. The first medium limited greening ability of callus grown in the light (3 milligrams per liter naphthalene acetic acid, 0.3 milligram per liter 2-isopentenylaminopurine, Murashige and Skoog salts, and 2% sucrose). The second medium encouraged chloro...

Here, efficient plantlet regeneration via somatic embryogenesis from Panax quinquefolium hairy roots was reported. Callus development was optimal on MS medium containing 2,4-dichlorophenoxyacetic acid (1 mg l) and -naphthalene acetic acid (1 mg l). After a 4-week growth, calli were transferred onto MS medium with different combinations of plant growth regulators (PGRs). The highest embryogenesi...

The GH3 family of acyl-acid-amido synthetases catalyze the ATP-dependent formation of amino acid conjugates to modulate levels of active plant hormones, including auxins and jasmonates. Initial biochemical studies of various GH3s show that these enzymes group into three families based on sequence relationships and acyl-acid substrate preference (I, jasmonate-conjugating; II, auxin- and salicyli...

The experiment was conducted to study the response of summer onion N2 and Naphthalene Acetic Acid (NAA). consisted two factors. Factor A: 4 levels Nitrogen viz. N0-0kg (control), N1-90kg ha-1, N2-110kg ha-1 N3-130kg B: NAA H0-0ppm, H1- 50ppm, H2-100ppm H3-150ppm. laid out in Randomized Complete Block Design with three replications. Results revealed that terms nitrogen, highest bulb diameter (4....

Holm oak populations have deteriorated drastically due to decline syndrome. The first objective of the present study was investigate use axillary budding and somatic embryogenesis (SE) propagate asymptomatic holm genotypes identified in disease hotspots Spain. Axillary achieved two out six tolerant from south-western region four Mediterranean region. Rooting shoots cultured on medium supplement...