Xanthine oxidase is a member of molybdoenzyme family and has catalytic role in purine degradation, metabolizing hypoxanthine and xanthine to uric acid with the generation of superoxides. In the present study, microtitre plate based colorimetric assay was used to screen the xanthine oxidase producing microorganisms from the soil treated with hypoxanthine with different concentrations (2mM, 4mM, ...

The reduction of nitroblue tetrazolium (NBT) by phagocytic leucocytes in peripheral blood has been examined in rabbits injected intratesticularly with Treponema pallidum, T. pallidum-free supernatant of orchitic testes, or normal rabbit testes supernatant. A significant (P less than 0.01) increase in NBT reduction was observed only in the infected animals.

We would like to present our experience of nasobiliary tube (NBT) transfer. A description of this technique, called the guidewire maneuver, follows (●" Video 1). The patient changes from the prone to the left lateral decubitus position while continuing to bite on the mouthpiece. The guidewire (Jagwire, 0.035in; Boston Scientific, Natick, Massachusetts, USA) previously used for endoscopic retrog...

Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching pO2's of less than 5 mm Hg fail to generate O2-, iodinate ingested particles, and stimulate glucose-1-14C oxidation through the hexose monophosphate shunt. The observation that anaerobic cells are incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supports the idea that NBT reducti...

The NADPH:O2 oxidoreductase catalyzing the respiratory burst in activated phagocytes from healthy individuals is not operative in phagocytes from patients with chronic granulomatous disease (CGD). In a microscopic slide test using the dye nitroblue tetrazolium (NBT), carriers of X-linked CGD can be recognized by a mosaic pattern of NBT-positive and NBT-negative cells, governed by the expression...

Since conflicting results have been reported on non-specific immune response in type 1 diabetes, this study evaluates polymorphonuclear neutrophil (PMN) functions in the infection free Long Evan diabetic rats (type 1) by using tests that include: polarization assay, phagocytosis of baker\'s yeasts (Saccharomyces cerevisiae) and nitroblue tetrazolium (NBT) dye reduction. Polarization assay showe...

The determination of gene expression patterns in three dimensions with cellular resolution is an important goal in developmental biology. However the most sensitive, efficient, and widely used staining technique for whole-mount in situ hybridization (WMISH), nitroblue tetrazolium (NBT)/5-bromo-4-chloro-3-indolyl phosphate (BCIP) precipitation by alkaline phosphatase, could not yet be combined w...

Hepatitis C virus (HCV) infection is the leading cause of liver transplantation in Western countries. Studies of HCV infection using cell culture-produced HCV (HCVcc) in vitro systems require quantification of infectious HCV virions, which has conventionally been performed by immunofluorescence-based focus-forming assay with manual foci counting; however, this is a laborious and time-consuming ...

Two new diterpenes were isolated from stems and leaves of Xylopia langsdorffiana, ent-atisane-7alpha,16alpha-diol (xylodiol) and ent-7alpha-acetoxytrachyloban-18-oic acid (trachylobane), along with the known 8(17),12E,14-labdatrien-18-oic acid (labdane). We investigated their antitumour effects on HL60, U937 and K562 human leukemia cell lines. We found that xylodiol was the most potent diterpen...

OBJECTIVES The fibrocytes, the mesenchymal cells and the colony forming units are included in the concept fibroblast-like, peripheral-blood circulating stem cells. The aim of this work was to establish the culture conditions. MATERIAL AND METHOD Peripheral blood mononuclear cells were isolated from 25 samples collected from the cephalic vein. These were cultured in McCoy's 5A enriched with 20...