The addition of 10(-5) M to 10(-3) M neomycin to incubations of subcellular fractions of guineapig cerebral cortex increased the labelling of phosphatidylinositol phosphate and decreased the labelling of phosphatidylinositol diphosphate by [gamma-32P]ATP. The effect was observed in all subcellular fractions tested and depended on the cationic form of the antibiotic. Similar effects on lipid lab...

The efficacy of neomycin in hepatic encephalopathy was highlighted3 as early as in 1957. The azotemia associated with aome fonru; of severe liver diseaseS militates against the use of neomycin in these circumstances6-8. The usefulness of ampicillin has been reported and the antibiotic has been suggested to be a satisfactory substitute9•1o. However, no study has directly compared the efficacy of...

The activity of antibiotics in vivo may be reduced by their interaction with constituents of blood or tissues, for example, the protein binding of penicillin and the binding of neomycin to mucus (Saggers and Lawson, 1966). During a study of the action of antibiotics on cutaneous bacteria in vitro, neomycin was found to precipitate long-chain fatty acids of the type present in skin. As neomycin ...

Differentiated primary rat hepatocyte cultures have been infected with retroviral vectors expressing human hypoxanthine/guanine phosphoribosyltransferase or the transposon Tn5 neomycin-resistance gene. Expression of the markers was detected only after infection of the cells during a short period of cell replication and transient dedifferentiation from days 1 to 5 of culture. Provirus integrated...