Species of coryneform bacteria (Corynebacterium glutamicum, Brevibacterium flavum, and B. ammoniagenes) utilize pretyrosine [beta-(1-carboxy-4-hydroxy-2,5-cyclohexadien-1-yl) alanine] as an intermediate in L-tyrosine biosynthesis. Pretyrosine is formed from prephenate via the activity of at least one species of aromatic aminotransferase which is significantly greater with prephenate as substrat...

Pathways of initial oxidation of n-alkanes were examined in two strains of Cladosporium resinae. Cells grow on dodecane and hexadecane and their primary alcohol and monoic acid derivatives. The homologous aldehydes do not support growth but are oxidized by intact cells and by cell-free preparations. Hexane and its derivatives support little or no growth, but cell extracts oxidize hexane, hexano...

Nicotinamide mononucleotide (NMN) is a key precursor of nicotinamide adenine dinucleotide and an important source cellular energy. It can prevent neuronal mitochondrial defects alleviate heart fibrosis. Strategies to improve NMN production have implications for human health. Through plasmid expression technology CRISPR/Cas9 technology, we engineered Escherichia coli efficient production. First,...

Best, Audrey N. (University of Georgia, Athens), and W. J. Payne. Preliminary enzymatic events in asparagine-dependent denitrification by Pseudomonas perfectomarinus. J. Bacteriol. 89:1051-1054. 1965.-The initial events leading to denitrification by Pseudomonas perfectomarinus grown anaerobically in the presence of NO(3) (-) were investigated. Conversion of asparagine to malic acid was demonstr...

NAADP (nicotinic acid-adenine dinucleotide phosphate) is a derivative of NADP (nicotinamide-adenine dinucleotide phosphate), which differs by the presence of a nicotinic acid instead of a nicotinamide moiety. This small structural difference makes NAADP one of the most powerful second messengers known, able to mobilize intracellular Ca2+ in a wide range of cellular models, ranging from inverteb...

Inosinic acid dehydrogenase was partially purified from sarcoma 180 cells. The concentrations of each reactant, inosine 5’-phosphate, nicotinamide adenine dinucleotide, and K+, altered both the apparent K, and Vmaxvalues for each of the other reactants. Enzymatic activity was inhibited by both products of the reaction, xanthosine S-phosphate and reduced nicotinamide adenine dinucleotide; the in...

The successful application of artificial neural networks toward the prediction of product distribution in electrophoretically mediated microanalysis is presented. To illustrate this concept, we examined the factors and levels required for optimization of reaction conditions for the conversion of nicotinamide adenine dinucleotide to nicotinamide adenine dinucleotide, reduced form by glucose-6-ph...

Human erythrocytes incubated in medium containing 50 mM glucose have increased intracellular sorbitol and fructose concentrations as compared with samples incubated with 5 mM glucose. Increased medium glucose concentration did not significantly alter total glucose consumption or lactate production. However, the intracellular lactate:pyruvate ratio rose, the concentrations of fructose diphosphat...

Ultraviolet light inhibits the photoreduction of 2,6-dichlorophenolindo-phenol or nicotinamide adenine dinucleotide phosphate with water as the electron donor (evolution of oxygen) but not the photoreduction of nicotinamide adenine dinucleotide phosphate with ascorbate as the electron donor. It inhibits photophosphorylation associated with either system. Experiments undertaken to test whether p...