Removal of iron from biologically important ligands by desferrioxamine (DF), in vitro, is exceedingly slow because of a kinetic barrier. This kinetic barrier can be overcome by a variety of a small molecular weight anions, of which nitrilotriacetate (NTA) is a prototype. We explored the effect of NTA on DF iron mobilization in vivo. Iron mobilization was increased and the effect was synergistic.

In an earlier report (1) it was shown that a band characteristic of the peptide bond appeared in the ultraviolet and that values of the molar absorption coefficient (em) at 205 mp could be used to characterize this band. It was shown, for a series of proteins, that the contribution of each peptide bond (ep) to the molar absorption coefficient is fairly constant, lying between 2500 and 2800. The...

Nanoparticle tracking analysis (NTA) was first applied to biologically active nanocomplexes to obtain concurrent information on their size, state of aggregation, concentration, and antigenic specificity in liquid. The subject of the NTA was an immunogenic complex (a candidate nanovaccine) comprised of spherical particles (SPs) generated by thermal remodeling of the tobacco mosaic virus and Rube...

In an earlier report (1) it was shown that a band characteristic of the peptide bond appeared in the ultraviolet and that values of the molar absorption coefficient (em) at 205 mp could be used to characterize this band. It was shown, for a series of proteins, that the contribution of each peptide bond (ep) to the molar absorption coefficient is fairly constant, lying between 2500 and 2800. The...

This study investigated the removal of sulfur and iron from shungite rocks through different methods after fine grinding: flotation, magnetic separation, microwave treatment, chemical leaching. In this work, first, a mineralogical was conducted. The carbon, silica, iron, compositions in as-received were 45.4%, 38.3%, 4.6%, 2.4%, respectively. grade 1.4% obtained. wet high-gradient separation at...

The kinetics of mineralization of 14C-labeled phenol and aniline were measured at initial concentrations ranging from 0.32 to 5,000 ng and 0.30 ng to 500 micrograms/g of soil, respectively. Mineralization of phenol at concentrations less than or equal to 32 ng/g of soil and of aniline at all concentrations began immediately, and the curves for the evolution of labeled CO2 were biphasic. The pat...

This work describes the synthesis and use of the chelator lipid, nitrilotriacetic acid ditetradecylamine (NTA-DTDA). This lipid is readily dispersed in aqueous media, both alone and when mixed with carrier lipids like dimyristoylphosphatidylcholine (DMPC). Fluorescence microscopic examination of membranes deposited from NTA-DTDA-containing liposomes shows that NTA-DTDA mixes uniformly with the ...

We optimized the expression and purification of outer membrane proteins SpaO LamB from Salmonella typhi. investigated various factors in processes, including use isopropyl β-d-1 thiogalactopyranoside (IPTG), imidazole, urea. First, PCR amplification was carried out on genes. The genes were then cloned pTZ57R/T, expressed pET28a vector transformed into Escherichia coli BL21 (DE3). Gene insertion...