Abstract The relative importance of introgression for diversification has long been a highly disputed topic in speciation research and remains an open question despite the great attention it received over past decade. Gene flow leaves traces genome similar to those created by incomplete lineage sorting (ILS), identification quantification gene presence ILS is challenging requires knowledge abou...

Abstract Jacob is a synapto-nuclear messenger protein that couples NMDAR activity to CREB-dependent gene expression. In this study, we investigated the nuclear distribution of and report prominent targeting envelope requires import. Immunogold electron microscopy proximity ligation assay combined with STED imaging revealed preferential association inner membrane where it directly binds LaminB1,...

Errors in the mRNP biogenesis pathway can lead to retention of mRNA in discrete, transcription-site-proximal foci. This RNA remains tethered adjacent to the transcription site long after transcriptional shutoff. Here we identify Sus1, Thp1, and Sac3 as factors required for the persistent tethering of such foci (dots) to their cognate genes. We also show that the prolonged association of previou...

The peripheral nuclear lamina, which is largely but not entirely associated with inactive chromatin, is considered to be an important determinant of nuclear structure and gene expression. We present here an inducible system to target a genetic locus to the nuclear lamina in living mammalian cells. Using three-dimensional time-lapse microscopy, we determined that targeting of the locus requires ...

The nuclear matrix appears to play an important role in developmental gene expression during osteoblast differentiation. To better understand this role, we examined nuclear matrix DNA-binding proteins that are sequence-specific and interact with the osteocalcin gene promoter. Multiple protein-DNA interactions involving two distinct nuclear matrix proteins occur within the 5' regulatory sequence...

Whether gene repositioning to the nuclear periphery during differentiation adds another layer of regulation to gene expression remains controversial. Here, we resolve this by manipulating gene positions through targeting the nuclear envelope transmembrane proteins (NETs) that direct their normal repositioning during myogenesis. Combining transcriptomics with high-resolution DamID mapping of nuc...

differentiation is an epigenetic process which is installed by changes of transcriptional programs over successive cellular divisions. a number of studies have reported the effects of biochemical modifications of chromatin (dna and chromatin proteins) on the regulation of transcription. although, these studies are able to explain how transcription of a given gene is regulated (toward activation...

Hysterothylacium fabri is commonly reported in various Mediterranean fish. However, there no data on the mitochondrial ribosomal RNA (rrnS) gene sequence of H. larvae Sea waters. Therefore, we aimed to reveal molecular characterization based rrnS current study. Firstly, fourth stage were identified at parasitological examination from Mullus barbatus Sea. Morphologically herein also supported by...